中文摘要：

为了进一步探讨提高PRRSV感染靶细胞滴度的有效方法，本研究采用脂质体介导方法，通过G418筛选稳定转染Marcl45细胞，并利用单个细胞克隆技术，获得了稳定超表达CDl51蛋白和（或）CDl63蛋白的Marcl45细胞株，经免疫荧光化学和Westem Blot鉴定证实，成功表达出基因重组猪CDl51蛋白和CDl63蛋白。在GMP生产车间将超表达CDl51、CDl63蛋白或CDl51／CDl63蛋白共表达的Marcl45细胞感染PRRSV，使病毒感染滴度提高了10倍以上。将上述收获的病毒液制备了灭活苗，疫苗效力超过部颁标准。

英文摘要：

In order to further explore the effective method to improve the PRRSV titer in infected target cells, the Marc145 cell lines stably over-expressing CD 151 and （or） CD 163 protein were established by liposome mediated method following G418 screen- ing and single cell cloning technology. The successful expression of recombinant porcine CD151 and CD163 proteins was identified by fluorescent immunoeytoehemistry and western Blot. Small scale experiment was conducted in the GMP production workshop and, through the over-expression of PRRSV receptor CD151, CD163 or CD151 and CD163 coexpressing, the virus titer in Marc145 cells infected with PRRSV increased by more than 10 times. Finally, the virus was harvested and the inactivated Vaccine was pro- duced. The vaccine ettleacy was qualified and higher than the standard promulgated by the Ministry of Agriculture.