中文摘要：

为了补充布氏石斑鱼细胞遗传学数据,并为石斑鱼染色体进化研究以及石斑鱼遗传育种等工作奠定基础,实验利用Giemsa染色、C-带和Ag-NORs方法对布氏石斑鱼的染色体核型以及异染色质和具有转录活性的核仁组织区在染色体上的分布位置和数量进行了研究。结果表明,布氏石斑鱼二倍体染色体数目为48,其染色体组由48条端部着丝粒染色体组成,绝大多数染色体的着丝粒位置呈异染色质带,仅在第24对染色体靠近着丝粒的位置检测到银染带,以上结果均表明,布氏石斑鱼属于较为原始的类群。

英文摘要：

Epinephelus bleekeri is important in commercial fisheries of tropical and subtropical seas.It has been broadly cultured in many Asian countries,such as China,Japan,Vietnam and Malaysia.Despite many studies on its culture techniques,the detailed cytogenetic data of this species is still lacking.To add E.bleekeri cytogenetic data and lay the foundation for grouper chromosome evolution and breeding studies,the karyotype of E.bleekeri was studied in this paper using multiple methods,including Giemsa staining,Ag-NORs and C-banding.The following results were obtained： E.bleekeri had a diploid chromosome number of 48 and its karyotype formula was 48t,NF=48,and sex chromosome was not observed;the con-stitutive heterochromatin was observed at the centromeric region on most chromosomes;but only a single pair of NORs was detected at the paracentromeric region of chromosome pair No.24.Based upon above results,it is concluded that E.bleekeri was relatively primitive among genus Epinephelus.