中文摘要：

目的：探讨多巴胺诱导白血病细胞系K562凋亡的作用机制。方法：先用与多巴胺受体相结合的带荧光的配基DP2785处理K562细胞后，分别采用紫外分光光度计和荧光分光光度计检测以及荧光显微镜观察，三方面证实K562细胞上是否存在多巴胺受体；进而检测细胞内cAMP水平；再通过受体阻断实验进行多巴胺受体亚型分析。结果：紫外分光光度计和荧光分光光度计检测结果以及荧光显微镜下观察结果显示，K562细胞上存在多巴胺受体；多巴胺可升高细胞内cAMP含量；受体阻断实验结果显示多巴胺作用于K562细胞，D1和D2受体均起作用。结论：多巴胺通过作用于K562细胞上D1和D2多巴胺受体，进而升高细胞cAMP含量起作用。

英文摘要：

Objective： To investigate the mechanism of the apoptosis-inducing effects of dopamine on K562 leukemia cells. Methods： K562 cells were treated with DP2785,the dopamine receptors were detected with fluorescence spectrophotometer, UV spectrophotometer and fluorescence microscope. the contents of cAMP in K562 cells were measured; and the subtypes of dopamine receptor on K562 cells were analyzed by receptor blocking. Results： The existence of dopamine receptors in K562 cells was demonstrated by fluorescence microscopy, UV spectrophotometer and fluorescence spectrophotometer. Dopamine enhanced the contents of cAMP in K562 cells. Dopamine receptors were blocked by both D1 and D2 antagonists. Conclusion： D1 and D2 dopamine receptors may be involved in dopamine-induced apoptosis of K562 cells,and dopamine can also increase the contents of cAMP in K562 cells.