中文摘要：

探讨具备不同拓扑结构的聚甲基丙烯酸甲酯（polymethylmethacrylate,PMMA）电纺纳米纤维细胞支架对于原代大鼠背根神经元（Dorsal Root Ganglion neurons,DRGn）神经突生长能力及形态的动态影响.构建具有随机或有序纤维空间排列形式的PMMA电纺纳米纤维;利用PMMA薄膜组作为对照,首先取PC12细胞作神经细胞培养的预实验;再于不同时间点分析在各组间DRGn神经突数量及长度的差异.结果显示PC12细胞和DRGn均能够在PMMA各型材料上贴壁及生长;培养第2天,培养在PMMA薄膜,随机及有序电纺纤维上的DRGn平均神经突数量（分别为3.3±1.6,2.9±1.2个和3.1±1.3个）和平均神经突长度（分别为143.7±45.2,134.2±38.7μm和138.4±40.6μm）均无显著差异;培养第3天,DRGn平均神经突数量（分别为6.7±3.1,6.3±2.7个和6.5±2.9个）仍无显著差异,但在有序PMMA电纺纤维上的DRGn能够生成更长的神经突（分别为（258.3±65.9,230.1±54.5）μm和（321.7±79.8）μm）（P〈0.05）;电纺纤维的拓扑结构对于DRGn神经突的生长方向具有明显影响,在有序纤维上的DRGn所生成的神经突能够和基质纤维的排列方向保持一致.研究结果提示有序排列的PMMA纳米纤维能够促进大鼠DRGn神经突的生长,其拓扑结构对于DRGn神经突具接触引导作用.

英文摘要：

To evaluate the value of polymethylmethacrylate（ PMMA） electrospun nanofibers with different topographic features as a scaffold for Dorsal Root Ganglion neurons（ DRGn） growth of rats,random and aligned PMMA electrospun nanofibers,and PMMA filmswere fabricated. All culture experiments were carried out five times using independently fabricated nanofibers and film samples. PC12 was used in the preliminary cell culture experiment,with PMMA film serving as the control,to reveal the difference of the growth patternsbetween the DRGn on random and aligned PMMA nanofibers.The results showed that PC12 cell and DRGn both could adhere to and grow on the nanofibers.On day 2 of culture,among PMMA films,random and aligned nanofibers,no difference was detected on average neurite number（ 3.3±1.6,2.9±1.2,and 3.1±1.3 respectively） and average neurite length（ 143.7±45.2,134.2±38.7,138.4±40.6） μm respectively）. On day 3,no difference was detected on average neurite number（ 6.7±3.1,6.3±2.7,6.5±2.9 respectively）,while DRGn on aligned nanofibers formed longer neurites（ 258.3±65.9,230.1±54. 5,321. 7 ± 79. 8） μm respectively）（ p 0. 05） comparing to the situation on the film and random nanofibers. Meanwhile,the topographic features of nanofibers could influence the alignment of DRGn neurites obviously,and on aligned nanofibers DRGn formed aligned neurites along the orientation of substrate nanofibers. The experiments reveal that aligned PMMA electrospun nanofibers could enhance the neurite growth of rats DRGn,and result in the alignment of neurites greatly.