中文摘要：

目的：探讨心理应激对大鼠实验性牙周炎的影响,观察高压氧（hyperbaric oxygen,HBO）治疗对心理应激相关牙周炎的疗效。方法：清洁级4周龄雄性Wistar大鼠80只,随机分为4组：（1）正常对照组;（2）牙周炎组：用浸有牙龈卟啉单胞菌株的丝线结扎左侧上颌第2磨牙牙颈部,复制实验性牙周炎模型;（3）单纯应激组;（4）牙周炎＋应激组。于实验后第9周停止应激刺激,对除正常对照组外其它各组大鼠每组选取4只进行HBO治疗。于实验后2、4和8周末分批处死动物,每组处死4只,于实验后10周末处死动物,每组处死8只。采血检测血糖浓度、血浆促肾上腺皮质激素（ACTH）、皮质类固醇和肾上腺素含量。测量术区的牙周附着情况,制作牙体牙周联合切片,观察牙周的组织学改变。结果：检测应激标记物的变化可见单纯应激组的血糖及血浆ACTH、皮质类固醇和肾上腺素含量在实验后第2、4周明显高于正常对照组和牙周炎组（P〈0.01）,第8周时血糖降至正常水平,但血浆ACTH、皮质类固醇和肾上腺素含量仍高于正常对照组和牙周炎组（P〈0.05）;牙周炎＋应激组在实验后第2、4和8周血糖及血浆ACTH、皮质类固醇和肾上腺素含量均高于正常对照组和牙周炎组（P〈0.05）;HBO治疗组牙周炎＋应激组血糖明显低于非治疗组（P〈0.01）,单纯应激组和牙周炎＋应激组血浆ACTH、皮质类固醇和肾上腺素水平显著下降（P〈0.01）。大体观察可见正常对照组及单纯应激组牙周附着位置正常;牙周炎组出现牙龈萎缩,附着丧失明显;牙周炎＋应激组附着丧失明显,根分叉暴露,HBO治疗后,牙龈水肿减轻,牙周袋变浅。单纯应激组与正常对照组在各时点的牙周附着水平的差异不显著（P〉0.05）;牙周炎＋应激组附着丧失程度在各时点均明显高于牙周炎组（P〈0.01）;HBO治疗结束后牙周炎组及牙周炎＋应激组牙?

英文摘要：

AIM： To investigate the role of chronic psychological stress on periodontitis and the effects of hy- perbaric oxygen （HBO） on periodontitis with psychological stress in rats. METHODS： Male special pathogen-free Wistar rats （ n = 80 ） were randomly divided into 4 groups ： （ 1 ） normal control group ; （ 2 ） experimental periodontitis group ： the pe- riodontitis model was induced by wrapping 3/0 silk ligature inoculated with Porphyromonas gingivalis around the left maxil- lary second molar of the rats ; （ 3 ） psychological stress stimulation group ; （4） periodontitis model with stress stimulation group. Psychological stress was removed at the 9th week after ligature, and 4 rats from each experimental group were ran- domly chosen for HBO treatment. The rats were sacrificed at the 2nd, 4th, 8th and 10th weeks after ligature. The levels of blood glucose, adrenocorticotropic hormone （ACTH） , corticosterone and adrenaline were measured as the stress markers. The histological changes of periodontal tissues were observed under microscope with HE staining. RESULTS ： The levels ofblood glucose, ACTH, enrtieosterone and adrenaline in psychological stress stimulation group and periodontitis with stress grqlup were significantly higher than those in control group and experimental periodontitis group at the 2nd and 4th weeks at： ter ligature （ P 〈 0. 05 ）. The levels of tire stress markers were significantly lower than those in untreated groups in tire 10th week afer 11130 （P 〈0. 01 ）. The sites of gingival attachment were normal in control group and psychological slress stimu- lation group. Periodontal pocket, and periodontal attachment loss （AL） were observed in experimental periodontitis group. The tissue danlage was much heavier in periodontitis model with stress stimulation group as the fureation of tooth wets ex- posed and the tissue damage was observed on both sides of the adjacent teeth. No significant difference of AI, between psy- ehologieal stress stimulation gro