中文摘要：

目的：建立同时测定玉泉丸中葛根素及甘草酸的HPLC方法。方法：采用Ultimate XB-C18 （4.6 mm ×250 mm，5μm）色谱柱；以不同浓度的乙腈（B）-0.2%醋酸溶液（A）为流动相，检测波长254 nm，流速1.0 mL·min^-1，柱温30 ℃。结果：葛根素及甘草酸均在1.56～200 μg·mL^-1浓度范围内具有良好的线性关系（r〉 0.999），平均加样回收率分别为97.2%和99.0%， RSD均小于3% （n=6）。结论：该法简便、快速，重现性好，可为玉泉丸质量控制提供定量分析方法。

英文摘要：

OIlJECTIVE To establish the HPLC method for the simultaneous determination of puerarin and glycyrrhizic acid in Yuquan pills. METHODS Chromatographic experiment was carried out on an Ultimate XB-C18 column （250 mm × 4. 6 mm, 5/zm） with a gradient elution composed of aeetonitrile （B） and 0. 2% acetic acid （A）. The flow rate was 1.0 mL·min^-1 and the detection wavelength was 254 nrru The column temperature was set at 30 ＇C. RESULTS The calibration curve showed good linearity over the range of 1.56 - 200/,g·mL^-1 for both of the ingredients determined （r〉0. 999）. The average recoveries for puerarin and glycyrrhizic acid were 97. 2% and 98. 0%, respectively with the corresponding RSDs below 3G （n = 6）. CON- CLUSION This method is simple, rapid, and reproducible. It can be used as a quantitative analysis method for quality control of Yuquan pills.