[目的]探讨锐齿栎叶片色素含量和光谱反射率之间的关系,确定无损、快速估算锐齿栎叶片色素含量的敏感反射光谱波段和光谱指数。[方法]在2个样地进行了2年的野外观测试验,于生长季内同步测定了锐齿栎叶片的光谱反射率和不同光合色素含量,分析了350 2 500 nm范围内光谱反射率和敏感光谱指数与叶片色素含量及比率之间的定量关系。[结果]锐齿栎叶片的光谱反射率随叶片色素含量呈明显的规律性变化,与Chl a、Chl b、Chl和Car的含量在可见光的绿光黄光及红边区域表现为显著或极显著的负相关性,与Car/Chl表现为极显著的正相关性;在近红外和短波红外区域,光谱反射率与色素的相关性不及可见光区。本文构建的色素敏感光谱指数ND(705,350)、ND(800,705)、m ND(800,705)和m PRI可以准确地估算锐齿栎叶片的Chl a、Chl b、Chl含量和Car/Chl比率。独立的试验数据检验表明估算值和实测值的拟合关系较好。[结论]ND(705,350)、ND(800,705)、mND(800,705)和mPRI可有效地估算锐齿栎叶片的色素含量及比率。
[Objective]To detect the relationship between the leaf pigment contents and spectral reflectance and to recommend useful hyperspectral wavebands and hyperspectral indices for nondestructive and quick estimation of pigment content of Quercus aliena var. acuteserrata. [Method]A field experiment was conducted in two plots over two years. The hyperspectral reflectance of 350 2 500 nm and different photosynthetic pigments content of leaves were systematically measured and analyzed. [Result]Spectral reflectance varied with different pigment contents. In green,yellow and red edge region of visible light region,the leaf spectral reflectance and Chl a,Chl b,Chl,and Car contents showed significant or highly significant negative correlation,the leaf spectral reflectance and Car / Chl showed highly significant positive correlation. In the near infrared and shortwave infrared region,the correlation between the spectral reflectance and the pigment was less than that in the visible light region. The established spectral indices sensitive to pigments,ND( 705,350),ND( 800,705),m ND( 800,705)and m PRI could be used to accurately predict the Chl a,Chl b,Chl contents and Car / Chl of leaves. The results of independent data verification showed that the fitting relationship of predicted value and the measured value was excellent. [Conclusion]The research indicated that the leaf pigment contents and ratio of Q. aliena var. acuteserrata could be predicted effectively with ND( 705,350),ND( 800,705),m ND( 800,705)and m PRI.