中文摘要：

应用NBS、DEPC、EDC、DIC、Ch．T、PMSF和DTT化学修饰剂对Aspergillus ficuum产内切菊粉酶和外切菊粉酶进行化学修饰，测定与其活性相关的氨基酸残基，结果表明，构成内切菊粉酶和外切菊粉酶活性中心的必需氨基酸残基均含有色氨酸和羧基氨基酸（谷氮酸或天门冬氨酸），组氨酸可能是酶活性中心的组成氨基酸。邹氏作图法进一步确认外切菊粉酶活性中心必需色氨酸残基数目为2，内切菊粉酶活性中心必需色氨酸残基数目为1。

英文摘要：

Exo-inulinase and endo-inulinase from Aspergillusficuum were subject to chemical modification of seven protein modification reagents and to identification of amino acid residues essential for enzyme activity. The results suggested that Nbromosuccinimide （NBS）, carbodiimide （EDC） and diethyl pyrocarbonate （DEPC） resulted in a great loss of endo- and exoinulinase activities, indicating that Trp, carboxyl amino acid （Glu or Asp） and His residues may be parts of the enzyme active site.s, respectively. Two Trp residues essential for exo-inulinase active site and one for endo-inulinase were confirmed by Tsou＇s method.