中文摘要：

[目的]研究秀丽线虫（Caenorhabdities elegans）ASH神经元介导硫酸铜躲避行为的分子机制。[方法]以硫酸铜为伤害性刺激物,考察其对TRPV通道基因ocr-2突变线虫的群体行为实验、个体行为实验、钙成像实验中的影响。[结果]ocr-2突变线对硫酸铜避指数降低了80%,在硫酸铜干滴刺激中后退位移仅为野生型的60%,并且其ASH神经元对硫酸铜刺激产生异常的约延时10 s的钙信号窄峰。[结论]OCR-2在线虫ASH神经元介导硫酸铜躲避行为中是必需的,能够介导硫酸铜刺激施加和撤除的钙信号峰的形成,同时还可能参与其他神经元与ASH神经元之间的抑制调控。

英文摘要：

[ Objective] Study the mechanisms of the ASH neurons mediating copper- avoidance behavior in Caenorhabdities elegans. [ Methods] In this study, using CuSO4 as stimuli we performed chemotaxis assay,dry drop test assay, and calcium ima- ging to examine the function of TRPV superfamily gene ocr- 2. [ Results] In chemotaxis assay ,ocr- 2 mutants decreased the a- voidance index by 80% ; in dry drop test, the displacement of ocr -2 mutants was only 60% as of wild type animals; the calci- um signals of ASH neurons in ocr -2 mutants presented a narrow peak with 10 s delay. [ Conclusion] These evidences suggest OCR -2 an indispensable role in the ASH neurons mediating CuSO4 avoidance behavior. The calcium signal in respond to the addition and removal of Cu2＋ stimuli is effected by OCR -2. Moreover, OCR -2 may also involve in inhibitory function in ASH neurons against ASH - regulating neurons.