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高寒草地禾草内生细菌B-401的鉴定及生物防治潜力评价

ISSN号：1004-5759
期刊名称：草业学报
时间：2014.6.20
页码：282-289
分类：S812.29[农业科学—草业科学;农业科学—畜牧学;农业科学—畜牧兽医] S432.42[农业科学—植物病理学;农业科学—农业昆虫与害虫防治;农业科学—植物保护]
作者机构：[1]草业生态系统教育部重点实验室甘肃农业大学草业学院甘肃省草业工程实验室中-美草地畜牧业可持续发展研究中心,甘肃兰州730070
相关基金：国家自然科学基金（No.31160122）和草业生态系统教育部重点实验室（甘肃农业大学）开放课题项目（No.CYzs-2011011）资助.
相关项目：东祁连山高寒草地禾草内生细菌的生物功能及机制研究

关键词：禾草内生细菌, 抑菌谱, 生物学功能, 鉴定, endophytic bacteria in grasses, inhibiting spectrum, biology function, identification

中文摘要：

为了明确高寒草地禾草内生细菌B-401的生物防治潜力，采用平板对峙法测定了其抑菌能力。抑菌谱表明，B-401对马铃薯坏疽病菌、马铃薯褐腐病菌、马铃薯炭疽病菌、马铃薯枯萎病菌、马铃薯干腐病菌、番茄早疫病菌、小麦根腐病菌、孜然根腐病菌和黄瓜枯萎病菌的抑制率分别达74．45％，71．57％，70．05％，48．01％，56．01％，60．25％，64．65％，45．01％和31．79％。在贮藏库中进行10倍液喷雾，对马铃薯坏疽病的防效达52．67％。生物学功能测定表明，该菌株在含有色氨酸和不含色氨酸的King培养基中分泌IAA分别为3．42和2．60mg／L，且具有固氮能力，无溶磷能力。对13401通过形态特征和生理生化测定，结合16SrDNA序列同源性分析，鉴定其为萎缩芽孢杆菌（Bacillusatrophaeus）。

英文摘要：

To study the biological control potential of B-401 endophytic bacteria in grasses on alpine grasslands, the determination of bacteriostatic ability was carried out by dual culture against plant pathogenic fungi. B-401 inhibited the growth of Phoma foveata , Stysanus stemonitis , Colletotrichum coccodes , Fusarium avenaceum , Fusarium oxysporum, Alternaria solani, Bipolaris sorokiniana and Fusarium solani by 74.45% , 71. 57%,70.05%, 48.01%, 56.01%, 60.25%, 64.65% and 45.01% respectively. The control efficacy of B-401 against potato gangrene was 52. 67 % when a 10 times dilution of fermentation broth was sprayed on potato tubers during the storage-period. The concentration of IAA secreted by B-401 in King＇s medium containing 100 mg/L tryptophan was 3.42 mg/L, and the concentration in the medium without tryptophan was 2.60 mg/L. B-401 possessed the capacity for nitrogen fixation but it did not have the capacity for phosphate solubilization. Based on morphology and physiological and biochemical characters, combined with 16S rDNA gene sequence analysis, B-401 was indentified as BaciLlus atrophaeus.

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