中文摘要：

目的 探究输血对黑色素瘤荷瘤小鼠TH1/TH2平衡及其细胞因子表达的影响.方法 构建黑色素瘤小鼠肿瘤模型,设新鲜血组与储存血组（30只/组）：经小鼠尾静脉分别输注新鲜全血（0 d）或储存血（15 d）0.2 mL,分别在输血后15、30 min与1、4、8h收集小鼠全血,用微量标本多指标流式蛋白定量技术（CBA）检测各时间点血浆中细胞因子TNF-α、INF-γ 、IL-2、IL-4、IL-10的表达水平;另设未输血组（6只/组）为对照组.结果 1）新鲜血组与对照组比较,输血后4 h TNF-α分泌水平（pg/mL）分别为9.14±0.83 vs 1.72±0.90（P＜0.01）;储存血组与对照组比较,输血后4 hIL-4和IL-10表达水平（pg/mL）分别为1.55±0.17 vs 0.29±0.08和5.4±1.39vs0.2±0.08（P＜0.01）.2）储存血组与新鲜血组比较,输血后1 h TNF-α和INF-γ表达水平（pg/mL）分别为3.67±1.76 vs 7.64±1.04（P＜0.05）和0.27±0.16 vs0.49±0.18 （P＜0.05）;输血后4 h IL-2的表达水平（pg/mL）分别为0.29±0.08 vs 0.89±0.21（P＜0.01）;输血后4 h IL-10的表达水平（pg/mL）分别为5.40±1.39 vs 2.38±0.54 （P＜0.01）.结论 输血可促进小鼠体内TH1和TH2细胞因子的表达,而相对于输注新鲜血,输注储存血组小鼠体内TH1细胞因子表达有所降低,而TH2细胞因子表达得到进一步促进,致TH1/TH2失衡,平衡向TH2方向偏移.

英文摘要：

Objective To investigate the effects of blood transfusion on TH1/TH2 balance and expressions of cytokines in melanoma-bearing mice.Methods The tumor model of melanoma mice was established.The fresh blood group and stored blood group were set up （30 mice/group）.Mice were transferred with 0.2 mL fresh blood （D0） or stored blood（D15） via tail vein.The whole blood in mice were collected at 15 minutes,30 minutes,1 hour,4 hours and 8 hours after blood transfusion respectively.The expression levels of cytokines （TNF-α,INF-γ,IL-2,IL-4,IL-10） in plasma at each time point were detected by cytometric beads array （CBA）.Control group was also set up without blood transfusion （6 mice/group）.Results 1） The secretion levels of TNF-α（pg/mL） were 9.14±0.83 in the fresh group and 1.72±0.90 in the control group respectively at 4 hour after blood transfusion （P〈0.01）.The expression levels of IL-4（pg/mL） were1.55±0.17 in the stored group and 0.29±0.08 in the control group respectively at 4 hour after blood transfusion （P〈0.01）.The expression levels of IL-10 （pg/mL） were 5.4±1.39 in the stored group and 0.2±0.08 in the control group respectively at 4 hour after blood transfusion （P〈0.01）.2） The expression levels of TNF-α（pg/mL） were 3.67± 1.76 in the stored group and 7.64± 1.04 in the fresh group respectively at 1 hour after blood transfusion （P〈0.05）.The expression levels of INF-γ（pg/mL） were 0.27±0.16 in the stored group and 0.49±0.18 in the fresh group respectively at 1 hour after blood transfusion （P〈0.05）.The expression levels of IL-2 （pg/mL） were 0.29±0.08 in the stored group and 0.89±0.21 in the fresh group respectively at 4 hours after blood transfusion （P〈0.01）.The expression levels of IL-10 （pg/mL） were 5.40± 1.39 in the stored group and 2.38±0.54 in the fresh group respectively at 4 hours after blood transfusion （P〈0.01）.Conclusion Blood transfusion promotes the expressions of TH1 and TH2 cytokines.Compared wi