用不同形态的镉培养液(氯化镉、硝酸镉、镉-EDTA、草酸镉和柠檬酸镉)对不同种类的蕨类植物(狗脊、舌厥、石松、瓦韦和石韦)进行培养。研究发现,镉在植物体不同部位的富集量是有差异的(富集量:根〉茎〉叶);对SEC-HPLC截取出的植物鳌合肽(PCs)研究表明,不同形态的镉能不同程度的诱发植物体中PCs的合成,从而PCs与Cd配位,降低镉的毒性,发现的配体主要为PC3,iso-PC4(βAla)和iso-PC4(βAla)。文章还研究了不同形态镉胁迫下的蕨类植物各部位中镉的分布情况,并且对植物体中经镉诱导形成的PCs-Cd配合物的形态进行研究,进而对其解毒机理进行探讨。
Different species of ferns (pyrrosia lingua,Lepisorus thunbergianus, Lycopodium japonicum, Elaphoglossum yoshinagae and Woodwardia japonica) were cultivated using different species of cadmium as cultivation solution. The results showed that there were differences in enrichment amounts of cadmium in different parts of plants (amounts in root〉in stem〉in leaves). And it was found that different species of cadmium could induce synthesization of plantchelated peptides (PCs) with different extents. And then PCs was coordinated with cadmium to decrease the toxicity from cadmium. There are some kinds of peptides ligands found in plants, which are mainly PC3, iso-PC3 (βAla) and iso-PC2(βAla). In the present paper, the distribution of cadmium in different parts of ferns was studied after the ferns were intimidated with different species of cadmium. And the species of PCs-Cd coordination compounds were also investigated to explain the detoxification mechanism aroused by it.