中文摘要：

调查 Heijiangdan 软膏的效果的目的(， HJD ) 在在 ⁶⁰ 公司光线在老鼠的导致放射的皮炎。有等级 4 放射皮炎的方法女 Wistar 老鼠由 ⁶⁰ 公司光线随机被划分成四个组(n=12 每组) ；对待 HJD， recombinant 人表皮的生长因素(rhEGF )-treated,对待 Trolox 、未经治疗的组，与一个否定控制组一起。在在处理以后的第 11 和第 21 天，在每个组的 6 只老鼠为评估被选择。superoxide dismutase (草皮) 的层次， malondialdehyde (MDA ) ，并且喂奶脱氢酶(LDH ) 用 spectrophotometric 方法被检测。成纤维细胞线粒体被传播电子显微镜学(TEM ) 观察。成纤维细胞生长因素 2 的表情(FGF-2 ) 和转变生长因素(TGF-1 ) 1 被西方的污点分析。在处理显示出重要差别以后，与未经治疗的组，草皮的层次， MDA 和 LDH 相比结果，在第 11 和第 21 天(P < 0.05 ) 。TEM 分析显示在未经治疗的组的成纤维细胞线粒体展出了胀大，， cristae 在 HJD 组，显得折断线粒体胀大是有限的，不平的 endoplasmic 蜂窝胃更出现了放松。与否定控制相比在未经治疗的组增加的 FGF-2 和 TGF-1 的表情组织(P < 0.05 ) 。在处理以后，在 HJD 组的 FGF-2， rhEGF 和 Trolox 的表达式显著地与未经治疗的组相比被增加(P < 0.05 ) ，或与否定控制组相比(P < 0.05 ) 。TGF-1 的表示显示出未经治疗、否定的控制组之间的重要差别(P < 0.05 ) 。HJD 和 Trolox 增加了 TGF-1 的水平，差别作为与未经治疗、否定的控制组相比显著(P < 0.05 ) 。结论 HJD 减轻氧化导致压力的损害，增加抗氧化剂活动，减轻成纤维细胞 mitochondrial 损坏，起来调整生长因素的表达式，并且在鼠标支持 mitochondrial 修理。

英文摘要：

Objective：To investigate the effects of Heijiangdan Ointment（黑绛丹膏,HJD） on oxidative stress in （60）Co γ-ray radiation-induced dermatitis in mice.Methods：Female Wistar mice with grade 4 radiation dermatitis induced by ~（60）Co γ-rays were randomly divided into four groups（n=12 per group）;the HJD-treated,recombinant human epidermal growth factor（rhEGF）-treated,Trolox-treated,and untreated groups,along with a negative control group.On the 11 th and 21 st days after treatment,6 mice in each group were chosen for evaluation.The levels of superoxide dismutase（SOD）,malondialdehyde（MDA）,and lactate dehydrogenase（LDH） were detected using spectrophotometric methods.The fibroblast mitochondria were observed by transmission electron microscopy（TEM）.The expressions of fibroblast growth factor 2（FGF-2） and transforming growth factor β1（TGF-β1） were analyzed by western blot.Results：Compared with the untreated group,the levels of SOD,MDA and LDH,on the 11 th and 21 st days after treatment showed significant difference（P〈0.05）.TEM analysis indicated that fibroblast mitochondria in the untreated group exhibited swelling and the cristae appeared fractured,while in the HJD group,the swelling of mitochondria was limited and the rough endoplasmic reticulum appeared more relaxed.The expressions of FGF-2 and TGF-β1 increased in the untreated group compared with the negative control group（P〈0.05）.After treatment,the expression of FGF-2,rhEGF and Trolox in the HJD group were significantly increased compared with the untreated group（P〈0.05）,or compared with the negative control group（P〈0.05）.The expression of TGF-β1 showed significant difference between untreated and negative control groups（P〈0.05）.HJD and Trolox increased the level of TGF-β1 and the difference was marked as compared with the untreated and negative control groups（P〈0.05）.Conclusion：HJD relieves oxidative stress-induced injury,increases the antioxidant activity,mitigates the