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A water-soluble fluorescence resonance energy transfer probe for hypochlorous acid and its application to cell imaging

ISSN号：1672-2922
期刊名称：《中华耳科学杂志》
时间：0
分类：Q51[生物学—生物化学] S859.799.1[农业科学—临床兽医学;农业科学—兽医学;农业科学—畜牧兽医]
作者机构：[1]Beijing National Laboratory for Molecular Sciences, Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, ChineseAcademy of Sciences, Beijing 100190, China
相关基金：This work was supported by the National Natural Science Foundation of China （20935005, 90813032, and 20875092）, National Basic Research Program of China （2010CB933502, 2011CB935800）, and the Chinese Academy of Sciences （KJCX2-EW-N06-01）.This work was supported by the National Natural Science Foundation of China （20935005, 90813032, and 20875092）, National Basic Research Program of China （2010CB933502, 2011CB935800）, and the Chinese Academy of Sciences （KJCX2-EW-N06-01）.

关键词：荧光共振能量转移, 次氯酸, 水溶性, 细胞成像, 应用, FRET, 探针, 罗丹明B, dansyl rhodamine B piperazinoacetohydrazide, water-soluble probe, hypochlorous acid, cell imaging,fluorescence resonance energy transfer

中文摘要：

水溶性的荧光回声精力转移(烦恼) 为 hypochlorous 酸(HOCl ) 的探查， dansyl 玫瑰精 B piperazinoacetohydrazide，被设计，综合并且描绘。当一个 FRET 施主和玫瑰精一半充当了一个 FRET 领受人，在探查的 dansyl 一半行动了。二一半被一张 HOCl 劈得开的活跃契约连接，并且这个连接器的劈开减少了 FRET 效率并且在 501 nm 增加了施主的荧光紧张。探查的水溶解度被 cationic 玫瑰精 fluorophore 的介绍与另外的探针相比改进。作为结果，探查能被用来与 210 mol/L 的一个线性范围和 80 nmol/L 的察觉限制在水的 biosystems 检测 HOCl (signal-to-noise = 3 ) 。探查成功地在 HeLa 房间被用于 HOCl 的荧光成像。

英文摘要：

A water-soluble fluorescence resonance energy transfer （FRET） probe for hypochlorous acid （HOCl）, dansyl rhodamine B piperazinoacetohydrazide, was designed, synthesized and characterized. The dansyl moiety in the probe acted as a FRET donor and the rhodamine moiety acted as a FRET acceptor. The two moieties were connected by a HOCl-cleavable active bond, and cleavage of this linker decreased the FRET efficiency and increased the fluorescence intensity of the donor at 501 nm. The water solubility of the probe was improved compared with other probes by introduction of the cationic rhodamine fluorophore. As a result, the probe could be used to detect HOCl in aqueous biosystems with a linear range of 2-10μmol/L and a detection limit of 80 nmol/L （signal- to-noise = 3）. The probe was successfully applied to fluorescence imaging of HOCI in HeLa cells.