中文摘要：

通过在无极核微乳液中水解乙烯基三乙氧基硅烷（TEVS）和3-氨丙基三乙氧基硅烷（APTES）,制备了疏水性光敏剂-2,9,16,23-四氨基酞菁锌负载的表面带有正电荷的二氧化硅纳米粒子（SiO2@ZnPc（NH2）4）。通过透射电镜（TEM）、Zetasizer Nano-ZS粒度仪（DLS）、紫外-可见分光光度计（UV-Vis）研究和表征了该纳米粒子的表面形貌、表面电荷、水溶性和稳定性。所合成的纳米粒子粒径约20nm,颗粒为规则的球形,粒径较均一,具有很好的分散性,平均ζ电位值为（28.8±2.79mV）,在714nm处有强吸收峰。此外,负载在纳米粒子中的ZnPc（NH2）4不易泄漏,增强了ZnPc（NH2）4的抗光漂白能力。以1,3-二苯基异苯并呋喃（DPBF）为探针分子,检测出SiO2@ZnPc（NH2）4可以有效产生单线态氧。通过测量纳米粒子与活细胞（HeLa,U251,PC-12）孵育后的细胞活度来检测对细胞的毒性,结果显示,纳米粒子浓度≤300mg/L时,未掺杂ZnPc（NH2）4的二氧化硅纳米粒子（SiO2-NH2）对细胞没有毒性;当其负载ZnPc（NH2）4后,对细胞没有显著毒性。

英文摘要：

Positive charged silica nanoparticles,entrapping hydrophobic photosensitizer 2,9,16,23-tetraamino-phthalocyanine zinc（ZnPc（NH2）4）,have been synthesized in the nonpolar core of micelles by hydrolysis of triethoxyvinylsilane（TEVS） and 3-aminopropyltriethoxysilane（APTES）.The water-soluble,stability,surface morphologies and charge were characterized using transmission electron microscopy（TEM）,dynamic light scattering（DLS） and UV-visible spectrophotometer.The as-prepared nanoparticles are highly monodispersed spheres with uniform diameter（about 20 nm）,and stable in aqueous system.Its average ζ potential value is 28.8±2.79 mV and exhibits strong Absorption peak at 714 nm.In addition,encapsulation of ZnPc（NH2）4 in the silica nanoparticles prevented ZnPc（NH2）4 from leaking and enhanced the anti-photobleaching.The ZnPc（NH2）4 entrapped-nanoparticles（SiO2@ZnPc（NH2）4） can efficiently generat singlet oxygen as measured by chemical probe 1,3-diphenylisobenzofuran（DPBF）.The toxicity of silica nanoparticles to cells has been investigated by the incubation of the ZnPc（NH2）4 entrapped-nanoparticles and the nanoparticles without ZnPc（NH2）4（SiO2-NH2） with living cancer cells（HeLa,U251,PC-12）.The experimental result reveals that both SiO2@ZnPc（NH2）4 and SiO2-NH2 particles have no significant toxicity when the concentration of the particles is below 300 mg/L.