在溶液培养条件下,利用膜不透性荧光染料PTS示踪技术研究了不同冬小麦基因型蒸腾与地上部钠(Na^+)积累间的关系。结果表明,蒸腾耗水量与地上二部Na^+积累的关系因盐胁迫浓度的不同而异。低盐(NaCl 150mmol/L)胁迫下,蒸腾耗水量对地上部Na^+的积累影响不大;高盐(NaCl 250mmol/L)胁迫下蒸腾耗水量与Na^+的积累量呈显著正相关。盐胁迫下,地卜部PTS含量与地上部Na^+的积累有着极显著的正相关,并随盐浓度的提高和胁迫时间的延长相关性增加。随盐胁迫浓度的提高,所有供试小麦品种的蒸腾耗水量和蒸腾速率下降,Na^+积累量、Na^+积累速率、胞内Na^+浓度和共质体流Na^+浓度等上升;随胁迫时间的延长,供试小麦品种的蒸腾耗水量、Na^+积累量、胞内Na^+浓度上升,共质体流中Na^+浓度、Na^+的积累速率明显下降。无论盐胁迫与否,NR9405(暖型)和小偃6号(中间偏冷型)的蒸腾耗水量和蒸腾速率均小于陕229(冷型)和RB6(冷型);蒸腾耗水量大,带走的热量多,是冷型小麦冠层温度低于暖型小麦的主要原因。在高盐环境中,质外体流对Na^+的吸收积累具有重要影响,质外体流越大,地上部的Na^+吸收积累越多。盐胁迫下高的蒸腾速率导致地上部盐分的快速积累对小麦耐盐是不利的。
Hydroponic experiments were performed with a kind of membrane-imperment fluorescent dye PTS, an apoplastic tracer, to investigate the relation between transpiration and sodium accumulation in the shoots of 4 different winter wheat genotypes. The results indicate that the relation varies with salt concentrations. Under lower salt (NaCl 150 mmol/L)stress, there is not any significant correlation between transpiration rate and sodium accumulation in the shoots, while, under higher salt(NaCl 250 mmol/L)stress, there is a significant correlation between them. The higher transpiration rate is, the more Na^+ accumulation is. There is also a significant correlation between the content of PTS and sodium accumulation, especially under higher salt concentration and longer stress time. As salt concentration increased, the transpiration rate and the transpiration velocity decrease, however, the amount and the rate of sodium accumulation, and the sodium concentrations in intracellular and symplastical fluids are all increased. The longer the salt stress time, the higher the transpiration rate, the sodium accumulation amount and the intracellular sodium concentration, the lower the sodium concentration and sodium accumulation in the symplastal flow. Whether there is salt stress or not, the transpiration velocity of warm winter wheat cuhivars NR9405 and Xiaoyan 6 are smaller than that cold winter wheat cultivars of Shaan 229 and RB6. These results indicate the lower canopy temperature of cold-type winter wheat is caused by the higher transpiration. Under higher salt stress, the bypass flow has great effect on sodium accumulation, the more the apoplastic leakage,and the more the shoot sodium accumulation. This means that higher transpiration is an adverse characteristic to wheat under salt stress.