中文摘要：

为研究菌株Dietzia sp.DQ12-45-1b降解十六烷产生的活性代谢产物,优化了该菌株产生的表面活性物质的提取和检测方法,分析了该菌株代谢十六烷不同时间产生表面活性物质的情况.结果显示,乙酸乙酯萃取法从培养液中回收代谢产物的效率最高,正己烷：氨水：异丙醇（2：1：4,V：V：V）的展层体系可均匀分开十六烷降解培养液萃取物.菌株DQ12-45-1b能够以十六烷为底物产生糖脂类表面活性物质,产量随培养时间延长逐渐增加.十六烷乳化层与培养液水相中表面活性物质含量比为4.16：1,经检测发现,水相萃取物中含有一种表面活性物质,而乳化层萃取物中含有两种表面活性物质.

英文摘要：

This experiment optimized the extraction and detection methods ofbiosurfactant produced by the strain Dietzia sp. DQI2-45-1b, and studied biosurfactant products of the strain growing on n-hexadecane for different cultural time. Ethyl acetate was the best extraction solvent with the highest metabolite recovery efficient. The mixture of hexane ： ammonia ： isopropanol - 2：1：4 （V：V：V） was used as the best solvent system in thin layer chromatography analysis. The strain DQI2-45-1b produced glycolipid biosurfactant, with the biosurfactant concentration of the culture broth positively correlated with the incubation time. The content ratio of crude extracts in emulsified hexadecane layer and aqueous medium was 4.16：1. A glycolipid was detected in the emulsified hexadecane layer but not in aqueous phase. Fig 5, Tab 1, Ref 15