从我国13个省市43个地区采集263份土壤样品及感染白僵菌的昆虫样品58份.采用稀释平板法和组织分离法进行白僵菌的分离及形态学鉴定,分离出白僵菌菌株154株,鉴定为白僵菌属2个种,分别是球孢白僵菌和布氏白僵菌,对其中的疑问菌株进行了核糖体DNA—ITS测序分析.所测菌株均为球孢白僵菌。
By means of dilute-separate technique and tissue-separate technique, 154 fungi strains were isolated from 263 soil samples and 58 stiff insects which infected by Beauveria from 43 regions of 13 provinces in China. The 154 Fungi strains were Beauveria bassicma and B. brongniartii by traditional classification. The strains were identified through DNA-ITS analysis, which had not distinct characteristic different from each other by traditional classification, and the results showed those strains were still B.bassianca