为了得到超声波-酶法提取蛹虫草培养基多糖的最佳工艺条件,利用Box—Benhnken中心组合试验及响应面(RSM)设计优化蛹虫草多糖的提取工艺,在单因素实验的基础上,建立了纤维素酶用量、酶解时间、超声时间、超声温度的4N素回归模型。确定了蛹虫草培养基多糖的最佳提取工艺为纤维素酶用量1.9%,酶解处理1.1h,在50℃下超声处理41min。在最佳条件下,蛹虫草多糖得率、为25.45%,
To establish the extraction technology of cellulase assisted by ultrasonic wave of Cordyceps militaris polysaccharides, he optimization was applied with Box-Behnken centerunited design and the method of response surface analysis (RSM). on the base of single factor investigation, effects of cellulase content, enzymolysis time, ultrasonic wave time and ultrasonic wave temperature as well as their interactions on yield of the polysaccharides were studied. The optimal extraction conditions were obtained as follows: the amount of cellulase 1.9%, enzymolysis time 1.1 h, ultrasonic wave temperature 50 %, ultrasonic wave time 41 min. Under the optimal condition, the yield of Cordyceps militaris polysaccharides was up to 25.45%.