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中文摘要：
以生长在开顶箱内的油松为试材,对高浓度臭氧（80 nmol.mol-1）条件下油松（Pi-nus tabulaeform is）针叶中超氧阴离子自由基（O2.-）产生速率、过氧化氢（H2O2）含量、超氧化物歧化酶（SOD）、抗坏血酸过氧化物酶、脱氢抗坏血酸还原酶、单脱氢抗坏血酸还原酶、谷胱甘肽还原酶活性与抗坏血酸（ASA）含量进行测定.结果表明：高浓度臭氧使O2.-产生速率提高,H2O2和MDA含量增加.ASA含量与SOD、抗坏血酸过氧化物酶、脱氢抗坏血酸还原酶、单脱氢抗坏血酸还原酶、谷胱甘肽还原酶活性在高浓度臭氧熏蒸的前期升高,随后下降并低于对照.说明生长季前期,油松抗氧化系统对高浓度臭氧存在适应性反应,但不能抵抗长期臭氧胁迫带来的氧化伤害.
英文摘要：
An open-top chamber experiment was conducted to study the effects of high concentration O3 （ 80 nmol · mol^-1 ） on the superoxide anion radical （ O2 ） generation rate, hydrogen peroxide （ H2O2 ） content, activities of superoxide dismutase （ SOD ）, ascorbate peroxidase （ APX ）, dehydroascorbate reductase （DHAR） , monodehydroascorbate reductase （MDHAR） and glutathione reductase （GR） , and ascorbic acid content in Pinus tabulaeformis leaves. Under high concentration O3 exposure, the superoxide anion radical generation rate and H2O2 and malondialdehyde （MDA） contents increased, while the ascorbic acid content and the activities of SOD, APX, DHAR, MDHAR, and GR increased in early growth season but decreased then to a level lower than the control, which illustrated that the antioxidant system of P. tabulaeformis did respond in an acclimation way in the early growth season, but could not bear the damage of long-term elevated O3 exposure.
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