中文摘要：

目的探讨系统性红斑狼疮（systemic lupus erythematosus,SLE）患者细胞因子信号转导蛋白抑制因子（suppres-sors of cytokine signaling,SOCS）1、SOCS2和SOCS3与细胞因子诱导的含SH2区域蛋白（cytokine-inducible SH2 containing pro-tein,CIS）的表达情况。方法收集SLE患者和健康志愿者各18例外周血标本,通过Ficoll-Hypaqu密度离心分离法分离外周血单个核细胞（peripheral blood monouclear cells,PBMCs）并提取总RNA,采用Real-Time PCR检测基因的表达并将表达水平与疾病临床指标做相关性分析。结果 SLE患者SOCS1的表达显著低于健康志愿者（P〈0.0001）,而表达水平与疾病临床指标无相关性,CIS的表达显著高于健康志愿者（P〈0.0005）,且表达水平与临床指标有显著相关性,但SOCS2（P=0.0575）和SOCS3（P=0.8034）在患者与健康者之间没有显著的差异。结论 CIS可以作为SLE潜在的诊断指标,可能参与SLE的发病机制。

英文摘要：

Objective The suppressors of cytokine signaling proteins,SOCS1,SOCS2 and SOCS3 and cytokine inducible SH2 protein（CIS） can regulate immune responses and cytokine signaling.The study aims to investigate the expression levels of CIS,SOCS1,SOCS2 and SOCS3 in peripheral blood mononuclear cells（PBMCs） of systemic lupus erythematosus（SLE） patients. Methods Blood samples of 18 SLE patients and 18 healthy volunteers were collected.Peripheral blood mononuclear cells were purified by Ficoll-Paque density-gradient centrifugation and total RNA was extracted.Real-Time PCR was used to detect the expression levels of CIS,SOCS1,SOCS2 and SOCS3. Results The expression of SOCS1 in SLE patients,which was not correlated with clinical indicators,was significantly lower than that in healthy volunteers（P 0.0001）.The expression of CIS in SLE patients,was significantly higher than that in healthy volunteers,and significantly correlated with clinical indicators（P 0.0005）.Differences between SLE patients and healthy volunteers were not statistically significant in SOCS2（P = 0.0575） and SOCS3（P = 0.8034）. Conclusions CIS can serve as a potential indicator of SLE and may be participated in the pathogenesis of SLE.