中文摘要：

采用双酶水解法提取乌骨鸡黑色素,研究碱液脱除蛋白,分离乌骨鸡黑色素的工艺条件。以腹腔注射D-半乳糖法建立小鼠氧化损伤模型,灌胃给予不同剂量乌骨鸡黑色素（500、250、100mg/kgbw）,观察其抗氧化活性。结果表明：碱液脱除蛋白分离乌骨鸡黑色素的最佳工艺条件为NaOH溶液（pH10）,温度60℃,时间1.5h,料液比1：10,提取4次。与模型组比较,乌骨鸡黑色素组小鼠血和肝脏中GSH-Px活力显著升高,MDA水平显著降低。乌骨鸡黑色素具有较强的体内抗氧化作用。

英文摘要：

Two-step enzymolysis using papain and protamex followed by protein removal using NaOH solution was adopted to prepare melanin from black-bone silky fowl meat. The effects of various NaOH solution treatment conditions on protein removal rate were studied by single-factor method. Subsequently, different doses （500, 250 and 100 mg/kg bw） of the melanin were administered by gavage to mouse models of oxidative injury induced by D-galactose to evaluate its antioxidant activity in vivo. The results showed that the optimal conditions for protein removal were as follows： ratio of material to liquid 1：10, pH 10.0, temperature 60 ℃, treatment time 1.5 h, and treatment number 4 times; a significant increase of GSH-Px activity and decrease of MDA content in blood and liver were found in melanin-treated groups in comparison with the model control group, indicating that melanin from black-bone silky fowl meat has significantly antioxidant activity.