中文摘要：

目的：建立测定大鼠脑组织中的单胺类神经递质含量的方法。方法：采用高效液相色谱-电化学法，以2，5-二羟基苯甲酸为内标，测定大鼠大脑皮层、小脑、海马组织、下丘脑、嗅球中以去甲肾上腺素（NE）和5-羟色胺（5-HT）为代表的单胺类神经递质的含量。色谱柱为DIKMA C18,流动相为缓冲盐溶液（醋酸钠、庚烷磺酸钠、乙二胺四乙酸二钠和柠檬酸）-甲醇（梯度洗脱），流速为1．0ml／min，工作电压为＋0．7V。结果：NE、5-HT的检测质量浓度线性范围分别为O．084-4．2μg／ml（r=0．9999）和0．005-0．25μg／ml（r=0．9991），平均加样回收率分别为98．85％（RSD=2．89％，n=3）和101．5％（RSD=2．41％，n=3），日内（n=5）和日间（n=3）RSD均不大于3％；NE在小脑中的含量最低[（0．343±0．14）mg／g]，在下丘脑中含量最高[（3．062±1．51）mg／g]；5-HT在小脑中的含量最低[（0．059±0．04）mg／g]，在大脑皮层中含量最高[（0．383±0．21）mg／g]。结论：本方法灵敏、简便、快速，可用于大鼠脑组织中NE、5-HT含量的测定。

英文摘要：

OBJECTIVE： To establish a method for the content determination of monoamines neurotransmitter in cerebral tissues of rats. METHODS： Using 2,5-dihydroxybenzoic acid as intemal standard, HPLC-ECD was adopted to determine the contents of monoamines neurotransmitter as norepinephrine （NE） and 5-hydroxytryptamine （5-HT） in pallium, cerebellum, hippocampus, hypothalamus and apophysis mamillaris of rats. The determination was performed on DIKMA C18 column with mobile phase consisted of buffer salt （sodium acetate, sodium heptanesulfonate, ETAs, citric acid）-methanol （gradient elution） at the flow rate of 1.0 ml/min. The voltage of electrode was ＋0.7 V. RESULTS： The linear range of NE and 5-HT were 0.084-4.2μg/ml （r=0.999 9） and 0.005-0.25 μg/ml （r=0.999 1）, respectively. The average recoveries were 98.85% （RSD=2.89%, n=3） and 101.5% （RSD= 2.41%, n=3）, respectively. RSD of intra-day （n=5） and inter-day （n=3） were no more than 3%. Content of NE in cerebellum was the lowest [（0.343 ±0.14） mg/g], and content of it in hypothalamus was the highest [（3.062 ±1.51） mg/g]. Content of 5-HT in cerebellum was the lowest [（0.059 ±0.04） mg/g], and content of it in pallium was the highest [（0.383 ±0.21） mg/g]. CONCLUSIONS： The method is sensitive, simple and rapid, and can be used for the content determination of NE and 5-HT in cerebral tissue of rats.