中文摘要：

在蚕的 flacherie 疾病被传染 flacherie 病毒(IFV ) 引起。IFV 依靠它 genomic RNA 的 5 区域到成员实现病毒的翻译和复制的主人相关的因素。为了识别，招待 IFV RNA 的 5 区域一定的蛋白质并且识别为它的函数重要的蛋白质，集体 spectrometry 被用来从用 RNA 被获得的蚕 midgut 摘录识别蛋白质标记 aptamer IFV RNA 的 5 区域。我们发现 325 蛋白质组(唯一的肽 2 )包括翻译相关的因素的 IFV RNA 的 5 区域一定( 16 个 ribosomal 子单元， 3 个真核细胞的开始因素子单元， 1 个延伸因素子单元和 6 潜力内部核糖体入口地点交易因素)，细胞骨架相关的蛋白质，膜相关的蛋白质，新陈代谢酶，并且另外的蛋白质。这些结果能被用来学习翻译，复制联系了在蚕与主人蚕并且到控制 flacherie 疾病交往的 IFV 的因素。

英文摘要：

The flacherie disease in the silkworm is caused by the infectious flacherie virus （IFV）. IFV relies on its 5＇ region of genomic RNA to recruit host-related factors to implement viral translation and replica- tion. To identify host proteins bound to the 5＇-region of IFV RNA and identify proteins important for its function, mass spectrometry was used to identify proteins from silkworm midgut extracts that were obtained using RNA aptamer-labeled 5＇ region of IFV RNA. We found 325 protein groups （unique peptide ≥2） bound to the 5＇ region of IFV RNA including translation-related factors （16 ribo- somal subunits, 3 eukaryotic initiation factor subunits, 1 elongation factor subunit and 6 potential internal ribosome entry site trans-acting factors）, cytoskeleton-related proteins, membrane-related proteins, etabolism enzymes, and other proteins. These results can be used to study the translation and replication related factors of IFV interacting with host silkworm and to control flacherie disease in silkworm.