中文摘要：

从黄河三角洲石油污染盐渍土中分离获得产生物表面活性剂的耐盐碱菌株,用于治理该地区的土壤污染问题。采用富集培养方法,分离得到4株优势菌,其中菌株BG降表面张力的能力最强。经生理生化实验和16SrRNA序列分析,鉴定菌株BG属于芽孢杆菌属（Bacillus sp.）。对该菌株产生物表面活性剂的性能进行研究,结果表明：菌株BG在37℃、180r/min下振荡培养48h,可将发酵液的表面张力由初始的58.4mN/m降低至25.1mN/m;温度对菌株BG产生物表面活性剂的影响较大,最适温度是37℃,此温度下菌株BG有良好的耐盐碱特性,显著降低发酵液表面张力。采用酸沉法提取了菌株BG产生的生物表面活性剂,鉴定为一种脂蛋白类物质,其中脂类和蛋白质质量分数分别为64.7%、35.3%。

英文摘要：

To remediate the petroleum contaminated saline soil in Yellow River Delta,four biosurfactant-produ-cing strains were isolated and studied. A halotolerant and alkali-resisting strain BG which produced biosurfactant more effectively was further studied. According to physiological and biochemical experiments and 16S rRNA sequence anal-ysis, this strain was classified to Bacillus sp.. The strain was studied and the results showed strain BG could reduce the surface tension of the fermentation liquid from 58.4 mN/m to 25.1 mN/m after 48 h fermentation under 180 r/min and 37 ℃. Temperature had significant impact on biosurfactant-producing ability of strain BG. Under the most appropriate temperature of 37 ℃ , BG strain owned good ability of halotolerant and alkali-resisting and reduced the surface tension evidently. The biosurfactant was extracted through acid precipitation method and identified as a kind of lipoprotein,with the proportions of lipid and protein were 64.7% and 35.3%, respectively.