中文摘要：

目的研究脐带间充质干细胞（UCMSCs）向胶质瘤干细胞的趋化迁移功能及其内在机制。方法原代分离培养并鉴定UCMSCs，用软、硬琼脂糖凝胶培养基质联合胶质瘤干细胞维持因子[表皮生长因子（ZGV）及碱性成纤维细胞生长因子（bFGF）]诱导胶质瘤干细胞限定在静息、增殖和分化状态，分别为软琼脂＋EGF＋bFGF组、硬琼脂＋EGF＋bFGF组、硬琼脂组，持续培养3组干细胞克隆球7d并绘制克隆球直径生长曲线；培养72h后流式细胞仪检测3组胶质瘤干细胞的细胞周期；免疫荧光染色检测3组胶质瘤干细胞克隆球CDl33、增殖细胞相关的核抗原（Ki67）和神经胶质纤维酸性蛋白（GFAP）的表达；Transwell实验检测UCMSCs向不同状态胶质瘤干细胞的迁移能力：酶联免疫吸附实验（ELISA）检测不同状态胶质瘤干细胞培养液中干细胞因子（SCF）、基质衍生因子-1（SDF-1）和血管内皮生长因子（VEGF）~表达；用培养72h的3组干细胞的条件培养液为培养基培养UCMSCs，Westernblotting检测UCMSCs中c-Kit、趋化因子受体-4（CXCR4）和血管内皮生长因子受体（VEGFR）蛋白的表达。结果培养2～7d时硬琼脂＋EGF＋bFGF组和硬琼脂组胶质瘤干细胞克隆球直径大于软琼脂＋EGF＋bFGF组，差异有统计学意义伴0．05）；细胞周期检测显示软琼脂＋EGF＋bFGF组、硬琼脂＋EGF＋bFGF组、硬琼脂组胶质瘤干细胞分别限定在静息、增殖和分化状态；软琼脂＋EGF＋bFGF组胶质瘤干细胞克隆球高表达CDl33[（95．79±5．31）％]，硬琼脂＋EGF＋bFGF组细胞克隆球高表达Ki67[（89．39±7．45）％]、GFAP[（63．49±16．54）％]；硬琼脂组细胞克隆球高表达GFAP[（97．36±3．48）％]；Transwell实验显示硬琼脂＋EGF＋bFGF组细胞迁移百分率高于软琼脂＋EGF＋bFGF组、硬琼脂组，差异有统计学意义（P〈0．05）；硬琼脂＋EGF＋bFGF组条件培养液中SCF、SDF．1和

英文摘要：

Objective To study the tropism of umbilical cord mesenchymal stem cells （UCMSCs） to glioma stem cells and their mechanism. Methods The isolated humanized UCMSCs were cultured and identified. The glioma stem cells were induced to remain at the resting, proliferation or differentiation states by soft or hard agar combined with stemness maintenance factors （epidermal growth factor [EGF] and basic fibroblast growth factor [bFGF]）： soft agar＋EGF＋bFGF group, hard agar＋EGF＋bFGF group, and hard agar group; clone spheres of the stem cells were cultured for 7 d, and then, the growth curve of clone spheres diameters was drawn; flow cytometry was used to detect the cell cycle of the stem cells 72 h after culture; CD133, Ki67 and GFAP expressions in the clone spheres of stem cells in the three groups were observed by immunofluorescent staining; the tropism aptitude of UCMSCs to glioma stem cells at different states was detected by Transwell dual chamber culture system; the protein expression levels of stem cell factor （SCF）, stromal-derived factor （SDF）-I and vascular endothelial growth factor （VEGF） in glioma stem cells were monitored by enzyme immunoassay （ELISA）; Western blotting was used to measure the protein expression levels of c-Kit, C-X-C chemokine receptor type 4 （CXCR-4） and VEGF receptor in UCMSCs. Results The diameters of clone spheres in the hard agar＋EGF＋bFGF group and hard agar group were significantly larger than those in the soft agar＋EGF＋bFGF group on the second and 7 d of culture （P〈0.05）; cell cycle analysis indicated that glioma stem cells in the soft agar＋EGF＋bFGF group, hard agar＋EGF＋bFGF group, and hard agar group were successfully remained at the resting, proliferation or differentiation states; high CD133 expression （ [95.79±5.31] %） was noted in the cells of soft agar＋EGF＋bFGF group, high Ki67 and GFAP expressions （[89.39±7.45 and 63.49±16.54] %） were noted in the cells of hard agar＋EGF＋bFGF group, and high GFAP expressi