中文摘要：

基于的 biomaterials 给在临床的应用潜在的伟人看了的镁(Mg ) 。然而，过多的 Mg ²⁺ 的细胞毒素的效果和从基于 Mg 的 biomaterials 的腐蚀产品，特别地他们神经原上的效果，很少被学习了。尽管生存能力测试最通常被使用，功能的评估极其被需要。这里，两甲基 thiazolyl tetrazolium (MTT ) 并且喂奶脱氢酶(LDH ) 试金被用来在 neuronal 生存能力上测试 Mg ²⁺ 和 Mg 摘录答案的效果。Microelectrode 数组(MEA ) 它提供长期，细胞外的 electrophysiological 的即时记录发信号在 vitro neuronal 网络，为有毒的效果习惯于测试。最小的有效集中(EC _{分别地，当时，从 MTT 和 LDH 试金的 Mg ²⁺ 的 min} ) 是 3  mmol/L 和 100  mmol/L EC _{从 MEA 试金获得的 min} 是 0.1  mmol/L。当文化暴露于 25%Mg 摘录答案时， MEA 数据揭示了 neuronal 网络活动的重要损失，没影响 neuronal 生存能力的集中。为与神经原评估基于 Mg 的 biomaterials 的 biocompatibility，测试的 MEA electrophysiological 比测试的基本房间生存能力是一个更精确的方法。

英文摘要：

Magnesium （Mg）-based biomaterials have shown great potential in clinical applications. However, the cytotoxic effects of excessive Mg2. and the corrosion products from Mg-based biomaterials, particularly their effects on neurons, have been little studied. Although viability tests are most commonly used, a functional evaluation is critically needed. Here, both methyl thiazolyl tetrazolium （MTT） and lactate de- hydrogenase （LDH） assays were used to test the effect of Mg2. and Mg-extract solution on neuronal viability. Microelectrode arrays （MEAs）, which provide long-term, real-time recording of extracellular electro- physiological signals of in vitro neuronal networks, were used to test for toxic effects. The minimum effective concentrations （ECmin） of Mg2. from the MTr and LDH assays were 3 mmol/L and 100 mmol/L respec- tively, while the ECmin obtained from the MEA assay was 0.1 mmol/L MEA data revealed significant loss of neuronal network activity when the culture was exposed to 25% Mg-extract solution, a concentra- tion that did not affect neuronal viability. For evaluating the biocompatibility of Mg-based biomaterials with neurons, MEA electrophysiological testing is a more precise method than basic cell-viability testing.