中文摘要：

目的：研究TRIM22蛋白对A型流感病毒复制能力的影响。方法：RT-PCR扩增人TRIM22全长编码序列并制备TRIM22真核表达质粒;通过绘制病毒生长曲线及直接做空斑的方法检测外源性表达TRIM22情况下,两种不同流感病毒株PR8（H1N1）和A/ST/364/2005（H3N2）在MDCK细胞中的复制能力。结果：成功构建TRIM22真核表达质粒;两种不同亚型流感病毒在过表达TRIM22的情况下,其在MDCK细胞中的生长曲线及空斑大小与阴性对照组相似。结论：TRIM22不能有效抑制不同亚型A型流感病毒的复制。

英文摘要：

Objective：To investigate the effect of TRIM22 protein on the replication of influenza A viruses.Methods：Full-length encoding sequence of human TRIM22 was cloned by RT-PCR and a recombinant plasmid expressing TRIM22 was generated.Two different influenza A viruses（PR8（H1N1）and A/ST/364/2005（H3N2））were used for the subsequent analysis of viral growth curves and plaque sizes in MDCK cells upon the exogenous expression of TRIM22 or not.Results：A eukaryotic plasmid expressing human TRIM22 protein was successfully constructed.Influenza A viruses exhibited the similar growth kinetics and plaque phenotypes in MDCK cells with or without the overexpressed TRIM22.Conclusion：TRIM22 has no obvious inhibitory effect on the replication of different influenza A viruses.