中文摘要：

本文研究了鸡、家兔、鲤鱼、蟾蜍4种实验动物血红蛋白（hemoglobin，Hb）携氧-释氧动力学过程，初步建立Hb携氧-释氧动力学研究方法，并探讨Hb携氧-释氧动力学过程与动物生存环境之间的关系。结果显示：4种动物Hb携氧动力学曲线均呈“S”形曲线特征，与传统的Hb氧解离曲线（oxygen dissociation curve，ODC）相似；同时不同动物Hb携氧-释氧动力学曲线也有各自特点，如鸡Hb释氧时间长达（1411±6）s；在Hb携氧-释氧曲线I阶段，鲤鱼上升斜率远大于家兔等。提示Hb携氧-释氧动力学曲线可反映不同动物Hb携氧效率的差异。与传统ODC参数P50相对应，由动力学曲线可得到Hb携氧动力学参数T50°T50是Hb达到50％氧饱和度所需时间，可直观反映Hb携氧效率的差异。4种实验动物Hb均有较稳定的T50,从大到小依次为：鸡、家兔、鲤鱼和蟾蜍。对Hb携氧动力学曲线与ODC综合分析，可得到Hb携氧效能参数E50,E50表示Hb达到50％氧饱和度所用时间与环境氧分压之间的关系，即E50（50％Sat，XPO^2，YT）。E50有可能成为全面评价Hb携氧效能的综合指标。

英文摘要：

The purpose of the present study was to examine the kinetic process of hemoglobin （Hb） carrying and releasing oxygen. Under the standard conditions （pH 7.4, Po2 20 mmHg, 20℃） the blood samples of chicken, rabbit, frog and carp were equilibrated in oxygen content analyzer with calibrated gas mixture A （0.5% CO2 and 99.5% N2）. Then the blood samples were exposed to gas mixture B （21% O2, 0.5% CO2 and 78.5% N2）. After equilibration, the blood samples were exposed to gas mixture A again. During the whole process, Po2 of blood samples was detected in real-time. The time spent in blood Po2 changing from 0 to 21 kPa was recorded carefully. The results indicated that the kinetic curve of Hb carrying oxygen presented a shape of＂S＂. It was similar to the Hb oxygen dissociation curve Crib ODC）. Based on the curve, T50, a new kinetic parameter, was established. T50 is the time of 50% O2 saturation of Hb. It can reflect the efficiency of Hb carrying oxygen. Through comparing of T50, the efficiency of Hb carrying oxygen among 4 species of animals was： frog 〈 carp 〈 rabbit 〈 chicken. In the phase I of lib carrying and releasing oxygen kinetic curve, the slope in carp was much larger than that in rabbit; the time [（1 411±6） s] of Hb releasing oxygen in chicken was longer than that in other 3 animals. These differences reflected the variety of efficiency of Hb carrying and releasing oxygen. In addition, the kinetic features of Hb carrying oxygen were likely to become an important index to evaluate the function of Hb carrying oxygen, especially in evaluating the ability of artificial blood substitute. On the basis of the analysis of the kinetic curve of Hb carrying oxygen and Hb ODC, another new important efficacy parameter E50 was proposed. E50 reflects the relationship between the time of 50% O2 saturation of Hb and environmental Po2. E50 can be used as a synthetic index to assess the efficiency of Hb carrying oxygen.