中文摘要：

目的：探索miRNA-182对细胞中电压门控钠离子通道1.7（Nav1.7）表达的影响及可能机制。方法：构建重组SCN9A 3＇UTR双荧光素酶报告基因载体并与miRNA-182抑制物、类似物或scramble共转染HEK293细胞,通过荧光素酶活性检测观察miRNA-182和SCN9A表达的变化。用谷氨酸钠刺激PC12细胞并转染miRNA-182类似物或抑制物,利用原位杂交、免疫荧光共检测以及蛋白免疫印迹方法检测细胞中miRNA-182和Nav1.7蛋白的表达。结果：miRNA-182能抑制SCN9A 3＇UTR在HEK293细胞中的表达。Nav1.7和miRNA-182在PC12细胞共表达。谷氨酸钠可使PC12细胞中Nav1.7表达增高,miRNA-182的表达降低（P〈0.05）;miRNA-182类似物能拮抗谷氨酸钠的刺激作用。结论：miRNA-182可负调控细胞内Nav1.7蛋白的表达。

英文摘要：

Aim： To investigate the effect of miRNA-182 on the expression of voltage-gated sodium channel 1. 7 （Navl. 7） in cells and its possible mechanism. Methods： Recombinant double luciferase reporter vector carrying SCN9A 3＇UTR was constructed and transfected into HEK293 cells binding with miRNA-182 agomir, antagomir or scramble, and the expressions of miRNA-182 and SCN9A were detected by luciferase assay system. Immunofluoreseenee, in situ hybrid- ization, and western blot were used to detect Navl. 7 and miRNA-182 in PC12 cells treated by glutamate and after transfect- ed with miRNA-182 agomir or antagomir. Results ： miRNA-182 could inhibit the expression of SCN9A 3＇UTR in HEK293 cells. Navl. 7 and miRNA-182 were co-expressed in PC12 ceils. The expression of Navl. 7 in PC12 cells with glutamate stimulation was significantly increased, while that of miRNA-182 was significantly decreased（P 〈 0.05 ） , miRNA-182 ag- omir could reverse the high expression of Navl. 7 protein in PC12 cells induced by glutamate. Conclusion： miRNA-182 could inhibit the expression of Navl. 7 protein in ceils.