中文摘要：

目的：探究肿瘤坏死因子α（tumor necrosis factorα,TNF-α）抑制剂XPro1595对大鼠脊神经结扎诱导的痛行为及脊髓背角白介素1β（IL-1β）、白介素6（IL-6）和TNF-α表达的影响。方法：运用L5脊神经结扎的方法建立神经病理性痛模型,通过鞘内注射给予TNF-α抑制剂（XPro1595）进行干预,采用Von Frey丝测定大鼠足底50%机械缩足反射阈值;运用Western Blot方法检测大鼠脊髓背角IL-1β、IL-6和TNF-α的表达情况。结果：（1）行为学结果显示：与对照组相比,脊神经结扎后大鼠表现出明显的机械性痛敏（P〈0.01）,且可被鞘内注射XPro1595有效改善（P〈0.01）;（2）Western Blot结果显示：与对照组相比,脊神经结扎组大鼠脊髓背角IL-1β、IL-6和TNF-α的表达显著升高,且可被鞘内注射XPro1595明显抑制（P〈0.01）。结论：鞘内注射XPro1595能够显著改善大鼠脊神经结扎诱导的神经病理性痛,其机制与抑制脊髓背角IL-1β、IL-6和TNF-α等炎性细胞因子的表达有关。

英文摘要：

Objective： To explore the effectof XPro1595,a TNF-α inhibitor,on spinal nerve ligation（ SNL）-induced nociceptive behavior and expressions of interleukin-1 beta（ IL-1β）,interleukin-6（ IL-6）,and tumor necrosis factor α（ TNF-α） after SNL in the spinal dorsal horn. Methods： L5 spinal nerve ligation was used to build neuropathic pain model. XPro1595 was administered intrathecally（ i. t.）. Mechanical allodynia was tested with Von Frey filaments. Expression of inflammatory cytokines（ IL-1β,IL-6 and TNF-α） were examined by Western Blot. Results：（ 1） Behavioral tests showed that the mechanical threshold of SNL-rats had significantly decreased comparedwith the sham group（ P〈0. 01）,and the administration ofX Pro1595 can elevated mechanical allodynia apparently（ P〈0. 01）;（ 2） Results of Western Blot indicated that expression of cytokines（ IL-1β,IL-6 and TNF-α etc.） in SNL group were up-regulated compared with the sham（ P〈0. 01）,and these changes were inhibited by XPro1595apparently（ P〈0. 05）. Conclusion： Intrathecal injection of XPro1595 can effectively improve the mehcanical allodynia induced by neuropathic pain through down-regulation of cytokines,such as IL-1β,IL-6 and TNF-α,etc.