中文摘要：

目的：探讨芪参益气滴丸预适应心脏微血管内皮细胞（CMEC）对心肌细胞（CMC）缺氧损伤的延迟保护作用。方法：应用Transwell小室建立CMEC与CMC共培养体系，实验分为心肌细胞组[包括正常对照组（Contr0l-cm）与损伤组（H／R-cm）]及共培养组[包括正常对照组（Contr0l）、损伤组（H／R）、中药预适应组（QSYQ）、缺氧预适应组（HPC）]。QSYQ组CMEC经过中药预适应1小时，HPC组CMEC经过缺氧40分钟复氧40分钟预适应，将各组CMEC小室插入CMC孔中，继续培养24小时后将H／R-cm组、H／R组、QSYQ组、HPC组同时进行缺氧24小时复氧2小时的缺氧损伤。以CCK-8、SOD评价细胞活力，以LDH、MDA评价细胞损伤程度，结果：与Contr0l-cm组相比，Contr01组心肌细胞的活力更好；与H／R组比较，H／R-cm组心肌细胞损伤更严重；QSYQ组、HPC组心肌细胞与H／R组比较均体现出细胞活力高，损伤程度小的优势，其差异有统计学意义（P〈0.05）。结论：共培养体系体现了CMEC与CMC的相互作用，而且共培养的细胞环境较单一细胞更接近心脏内环境条件；中药芪参益气滴丸预适应的CMEC与经过缺氧预适应的CMEC对心肌细胞长时间缺氧损伤具有同样的延迟保护作用。

英文摘要：

Objective： To discuss QiShen YiQi dripping pills （QSYQ）-induced delayed preconditioning of car- diac microvascular endothelial cells（CMEC） to hypoxic injury of myocardial cells. Methods： Transwell chamber was used to establish CMEC and CMC co-culture system, the experiment could be divided into myocardial cells groups （containing normal control group （Control-cm） and injury group （H/R-cm） and co-culture group [including normal control group （Control）, injury group （H/R）, herbal preconditioning group （QSYQ）, hypoxic preconditioning group （HPC）]. After QSYQ preconditioned CMEC in QSYQ group for one hour, hypoxia-reoxygenation preconditioned CMEC in HPC group for each 40 minutes, transwell chambers of different groups were inserted in CMC wells, cul- tured for 24 hours, H/R-cm group, H/R group, QSYQ group and HPC group were undergoing hypoxic injury. CCK-8 and SOD were used to assess cellular viability, LDH and MDA to assess the degrees of cellular injury. Results： Com- pared with Control-cm group, the viability of myocardial cell was better; compared with H/R group, the injury of myocardial cells in H/R-cm group was worse. Myocardial ceils in QSYQ group and HPC group presented the advan- tages of higher cellular viability and less harm when they were compared with H/R group, the difference showed sta- tistical meaning （P〈0.05）. Conclusion： Co-culture system could reflect the interaction between CMEC and CMC, and co-culture system is closer to internal environment of the heart compared with single cell; QSYQ and HPC pre- conditioned CMEC 13ossess the same delayed protection to hypoxic injury of myocardial cells.