Streptomyces rubrogriseus HDZ-9-47,从 Meloidogyne spp 的鸡蛋孤立,作为 Meloidogyne n 的一个潜在的 biocontrol 代理人被评估在下面在 vitro 和保护的地。显微镜的观察显示出那 HDZ-9-47 M 的寄生于的鸡蛋。在 7 天以内的 n。在 vitro,文化引起 M 的秒阶段少年(J2s ) 的 97.0% 死亡的 HDZ-9-47 过滤。n 并且禁止的超过 50% 鸡蛋孵化。在这域里,与在处理的控制,根结索引和 J2s 密度相比淋湿包含的肉汤 10 个 12 HDZ-9-47 孢子被 51.1 和 80.7% 分别地在 90 天帖子移植减少,它在另外的应用程序剂量和方法比那好。另外,减小与 biofumigation 根结索引和 HDZ-9-47 的联合申请的处理的 J2s 密度评价分别地,是 87.1 和 91.0% 比独自使用的 HDZ-9-47 或 biofumigation 或 fosthiazate 处理的任何一个好。并且西红柿产量也增加了 16.1% 。一起,我们的结果建议 HDZ-9-47 能是 M 的一个有效 biocontrol 代理人。与卷心菜残余 biofumigation 相结合的 HDZ-9-47 的 n,和那应用是为 M 的一种有希望、持续的选择。n 控制。
Streptomyces rubrogriseus HDZ-9-47,isolated from eggs of Meloidogyne spp.,was evaluated as a potential biocontrol agent of Meloidogyne incognita under in vitro and protective field.Microscopic observations showed that HDZ-9-47 parasitized eggs of M.incognita within 7 days.In vitro,the culture filtrate of HDZ-9-47 caused 97.0%mortality of second-stage juveniles(J2s)of M.incognita and inhibited more than 50%egg hatching.In the field,compared with the control,the rootknot index and J2s density in the treatment of drench the broth contained 1012 HDZ-9-47 spores were respectively reduced by 51.1 and 80.7%at 90 days post transplantation,which were better than that in other application doses and methods.In addition,reduction rates of root-knot index and J2s density of the treatment of combined application of HDZ-9-47 with biofumigation was 87.1 and 91.0%,respectively,better than either of HDZ-9-47 or biofumigation used alone or fosthiazate treatment.And tomato yield also increased by 16.1%.Together,our results suggest that HDZ-9-47 could be an effective biocontrol agent of M.incognita,and that application of HDZ-9-47 combined with cabbage residue biofumigation was a promising and sustainable option for M.incognita control.