中文摘要：

目的：研究铁皮石斛原球茎诱导与增殖的最适培养基配方。方法：以铁皮石斛种胚为材料,以MS培养基为基本培养基,研究不同植物激素6-苄基腺嘌呤（6-BA）、萘乙酸（NAA）、2,4-D（2,4-二氯苯氧乙酸）和激动素（KT）或其组合及马铃薯提取液（PE）、香蕉提取液（BE）、苹果提取液（AE）和椰乳（CM）等有机添加物对铁皮石斛原球茎诱导和增殖的影响。结果：6-BA与NAA配比不利于种胚萌发,萌发度均低于MS培养基;添加10%的PE、CM有利于原球茎诱导;2,4-D对原球茎的生长和增殖不利,一定浓度的BA、KT和NAA有利于原球茎的增殖;KT浓度为1.0 mg/L时,原球茎40 d增殖倍数最高,达9.0;PE、CM、AE都能不同程度的促进原球茎的增殖,以10%的CM效果最好。结论：适宜铁皮石斛原球茎的诱导培养基为MS＋10%CM＋1.0 g/L AC;适宜铁皮石斛原球茎增殖的培养基为MS＋1.0mg/L KT＋0.2mg/L NAA＋10%CM。

英文摘要：

Objective： To study on the optimal medium ingredients for PLB induction and proliferation of Dendrobium officinale.Methods： Seed embryos of Dendrobium officinale were cultivated in MS medium as the basic medium,along with different plant hormones like 6-BA,NAA,2,4-D and KT or their combinations added with organic additives like PE,BE,AE and CM.Results： BA and NAA combination was not conductive to germination and the germination ratio was even lower than that of MS medium;10% PE and CM was beneficial to PLB induction;2,4-D was not conductive to growth and proliferation;A certain concentration of BA,KT and NAA was beneficial to PLB proliferation;KT at 1.0 mg/L recorded the highest 40 d PLB proliferation times at 9.0;PE,CM and AE could promote the PLB proliferation at different levels,among which 10% CM was the most effective.Conclusion： The optimized medium ingredients suitable for PLB induction are MS＋10%CM＋1.0g/L AC;The optimized medium ingredients suitable for PLB proliferation are MS＋1.0 mg/L KT＋0.2 mg/L NAA＋10%CM.