中文摘要：

研究沙眼衣原体（Chlamydia trachomatis,Ct）血清型LGV L2在体外培养的繁殖规律及其影响因素,确定血清型LGV L2在体外培养的最佳生长发育条件。用沙眼衣原体血清型LGV L2分别感染HEp-2细胞、HeLa229细胞、HepG-2细胞、SGC-7901细胞和Vero细胞,在荧光显微镜下计数包涵体形成单位（inclusion fig-ure unity,IFU）和观察培养不同时间后包涵体的形态,比较不同细胞对血清型LGV L2的敏感性及血清型LGVL2在不同细胞内的生长情况。同时分别设DEAE葡聚糖处理组与未处理组,含放线菌酮培养组与不含放线菌酮培养组,比较培养12、24、36和48 h后血清型LGV L2包涵体形态、IFU和Real-Time PCR定量检测血清型LGV L2的核酸量,判断DEAE葡聚糖和放线菌酮对沙眼衣原体血清型LGV L2生长的影响。在感染20 h后,显微镜下观察发现HEp-2、Vero、HepG-2、HeLa和SGC-7901细胞均不同程度肿胀,5种细胞内均可见包涵体,大约40~48 h后包涵体占据整个胞浆。IFU计数和Real-Time PCR结果显示5种细胞中HeLa细胞感染率最高,HepG-2细胞感染率最低,血清型LGV L2在HeLa细胞中生长速度最快。荧光显微镜下计数IFU,发现DE-AE葡聚糖预处理组和对照组中血清型LGV L2的感染率和生长发育没有明显区别,而含放线菌酮培养组中各细胞内血清型LGV L2生长速度较对照组快,Real-Time PCR检测结果显示放线菌酮组各细胞内血清型LGVL2核酸量较对照组高。血清型LGV L2在HeLa细胞中的感染率最高,DEAE葡聚糖对血清型LGV L2的感染没有明显影响,而体外培养时添加放线菌酮有利于血清型LGV L2的生长发育。

英文摘要：

The propagation pattern and influential factors of trachoma（Chlamydia trachomatis） serotype LGV L2 in vitro were studied and confirmed the optimization of its growth conditions in vitro.HEp-2,HeLa229,HepG-2,SGC-7901 and Vero cells were respectively infected with C.trachomatis LGV L2,then stained with fluorescent antibody,and inclusion bodies were observed under fluorescent microscope and counted the inclusion figure unity（IFU） after different times＇ cultivation and compared the sensitivity of different cells to serotype LGV L2 and the growth of serotype LGV L2 in different cells.Meanwhile,DEAE-dextran treated group,untreated group,and cycloheximide containing group and cycloheximide non-containing group were set up respectively,then compared the shape of LGV L2 inclusion bodies,the quantity of IFU and the quantity of nucleic acid of serotype LGV L2 by Real-Time PCR quantity detection 12,24,36 and 48 h after cultivation to determine the effect of DEAE-dextran and cycloheximide on the growth of Chlamydia trachomatis serotype LGV L2.The results showed that 20 h after the infection,it was found under microscope that HEp-2,Vero,HepG-2,HeLa,and SGC-7901 and cells swelled in varying degree,and the inclusion bodies could be found in the five kinds of cell,and the entire cytoplasm was occupied with inclusion bodies about 40～48 h after the infection.And the results of IFU counting and Real-Time PCR showed that HeLa cells were in the highest infection rate among the 5 cells,while HepG-2 cells were in the lowest infection rate,and serotype LGV L2 grew the fastest in HeLa cells.It was found IFU counting under fluorescent microscope that the infection rate and growth of serotype LGV L2 between DEAE-dextran pretreated group and the control group,while the infection rate and growth in cells of serotype LGV L2 containing cycloheximide cultured group grew faster than the control group and Real-Time PCR test results showed the content of nucleic acid of serotype LGV L2 in cells of cycloheximide group was higher than the