中文摘要：

目的观察附子对高糖培养下施万细胞中Krox20-Oct6途径及其调控的髓鞘蛋白的影响,以揭示附子治疗糖尿病周围神经病变的机制。方法将施万细胞分为以下6组：（1）正常组（低浓度葡萄糖）;（2）对照组（甘露醇）;（3）模型组（高浓度葡萄糖）;（4）附子水提物高、中、低剂量组（10μg/mL,1.0μg/mL,0.1μg/mL）。常规培养3天后,采用实时荧光定量PCR法检测各组细胞Oct6、Krox20、髓鞘碱性蛋白和髓鞘蛋白Z mRNA的表达水平,采用Western blot法检测各组细胞的Oct6和Krox20蛋白的表达水平,采用免疫荧光法检测各组细胞的髓鞘碱性蛋白和髓鞘蛋白Z蛋白的表达水平。结果 PCR结果显示,与正常组相比,模型组中Oct6、Krox20、髓鞘碱性蛋白和髓鞘蛋白Z m RNA表达水平下降;与模型组比,附子水提物各剂量组中Oct6、Krox20、髓鞘碱性蛋白和髓鞘蛋白Z m RNA表达水平上升。Western blot结果证明,与正常组相比,模型组中Oct6蛋白、Krox20蛋白表达水平下降;与模型组比,附子水提物各剂量组中Oct6蛋白、Krox20蛋白表达水平上升。免疫荧光结果提示,与正常组相比,模型组中髓鞘碱性蛋白蛋白及髓鞘蛋白Z蛋白表达水平下降;与模型组比,附子水提物各剂量组中髓鞘碱性蛋白蛋白及髓鞘蛋白Z蛋白表达水平上升。结论高浓度葡萄糖通过抑制Krox20-Oct6途径使施万细胞髓鞘蛋白生成能力下降,而附子可能通过Krox20-Oct6途径促进髓鞘蛋白的形成,从而改善糖尿病周围神经病变。

英文摘要：

Objective To investigate the Krox20-Oct6 signaling pathway in Schwann cells induced by high glucose and to analyze the effect of aconiti lateralis radix praeparata on the formation of myelin sheath, and then clarify the mechanisms underlying the therapeutic effect of aconiti lateralis radix praeparata in diabetic peripheral neuropathy. Methods Schwann cell were divided into six groups. In the control group, the cells were supplemented with normal cell culture medium. In the mannitol group, the cells were fed with normal glucose plus mannitol. In the model group, the cells were supplemented with high glucose medium. In the other group, the cells were treated with high glucose medium plus different concentrations of aconiti lateralis radix praeparata （0. 1 μg/mL, 1. 0 μg/mL and 10. 0 μg/mL）. After three days, Real-time PCR was used to detect the expression of Oct6, Krox20, myelin basic protein and＆nbsp;myelin protein zero mRNA. Western blot was used to detect Oct6 and Krox20 protein expression. Myelin basic protein and myelin protein zero protein expression was detected by immunofluorescence. Results PCR results showed that compared with the control group, the model group showed lower expression of Oct6, Krox20, myelin basic protein and myelin protein zero protein. In comparison to the model group, aconiti lateralis radix praeparata （0. 1 μg/mL, 1. 0 μg/mL and 10. 0 μg/mL） increased the expression of Oct6, Krox20, myelin basic protein and myelin protein zero protein. Western blot results indicated that compared with the control group, the model group showed lower expression of Oct6 and Krox20. In comparison to the model group, aconiti lateralis radix praeparata （ 0. 1 μg/mL, 1. 0 μg/mL and 10. 0 μg/mL） increased the expression of Oct6 and Krox20. Immunofluorescence results showed that compared with the control group, the model group showed lower expression of myelin basic protein and myelin protein zero protein. In comparison to the model group, aconiti lateralis radix praeparata （0. 1 μg/