中文摘要：

目的探索豚鼠耳蜗基底膜振动测试的方法。方法用戊巴比妥钠腹腔注射麻醉豚鼠后,腹侧切口,逐层切开皮肤,分离肌肉,可见听泡位于豚鼠下颌骨内侧深面,钝性分离听泡表面的肌肉组织,暴露听泡,用电钻将听泡打开,可见螺旋形耳蜗结构,辨认基底膜所在的部位。用电钻在需要开孔的骨壁上轻磨一个环形的凹陷,待足够薄弱的时候用尖头的刀片挑开骨片。用具有微细尖端的不锈钢针蘸取少量反光微珠,放置在基底膜上。然后用激光多普勒测振仪（laser doppler vibrometer,LDV）测量基底膜在外耳道纯音激励下的振动。结果用该方法测量了豚鼠耳蜗基底膜在不同频率声波激励下的响应曲线。结论该方法可进行豚鼠耳蜗基底膜振动的离体实验,但活体的振动测试尚需进一步的探索。

英文摘要：

Objective To explore a method to measure the vibration in basilar membrane of guinea pig′s cochlea. Methods Guinea pig was under pentobarbital anesthesia by intraperitoneal injection throughout the experiments.We made a ventrolateral incision and dissected the muscles.Then the bulla could be seen at the deep side of the mandible.The bulla was exposed by scraping the muscles on its surface,and was opened by electric drill.After the location of the basilar membrane was distinguished,a circle ditch was drilled gradually on corresponding bony wall.Then a fine hole of the bony wall could be done by openng this small piece isolated bony wall with a pointed scalpel.The reflected glass beads were placed on the basilar membrane using a pointed stainless needle.Then the laser doppler vibrometer（LDV）was used to record the vibration in basilar membrane under pure tone stimulation from the outer ear. Results We measured the response curve of basilar membranem in guinea pig′s cochlea under sound stimulation of different frequencies. Conclusions This method can be used to measure the vibration in basilar membrane of guinea pig′s cochlea in vitro,while in vivo test may need further research.