中文摘要：

目的：探讨2型糖尿病患者的口腔念珠菌感染情况及其唾液人类8防御素（HBD-2、HBD-3）的表达水平和相互关系。方法：纳入确诊6个月以上近期病情没有变化的2型糖尿病患者39例为实验组及健康者40例为对照组，分别采集两组未刺激全唾液、含漱液，进行口腔念珠菌培养鉴定计数，并采用酶联免疫吸附法检测唾液HBD-2及HBD-3表达水平。结果：实验组中27例（69．2％）念珠菌培养呈阳性，明显高于对照组（20．0％），均以白色念珠菌为主。实验组唾液HBD-2和HBD-3的水平中位数分别为0．045μg／L和8．061μa／L，均明显高于对照组（0．024μg／L和0．832μg／L），两组比较差异均有统计学意义（P〈0．05）；口腔念珠菌负荷与唾液HBD-3的水平呈正相关（rs=0．245，P〈0．05）；唾液HBD-2与HBD-3的水平呈正相关（rs=0．370，P〈0．01）。结论：2型糖尿病患者的口腔念珠菌负荷普遍较高，分离株以白色念珠菌为主，其唾液中HBD-2、HBD3的水平均升高，并与口腔念珠菌负荷呈正相关关系。

英文摘要：

Objective： The aim of this study was to explore the levels of HBD-2 and HBD-3 protein in saliva of NIDDM patients and their relationship with oral candidiasis. Methods： 39 NIDDM patients who were di- agnosed for over 6 months without recent fluctuation and 40 healthy controls were recruited. Oral rinse and unstimulated saliva were collected. Oralcandida were cultured and calculated and the concentration of HBD 2,-3 were detected by enzyme-linked immunosorbent assay. Results：In NIDDM group, oralcandida were i- solated from 27 of the 39 patients （69.2%）, which were mainly identified as C. albicans. While in healthy controls, only 20.0G of oral candida culture was positive. The median concentrations of HBD-2 and HBD- 3 in NIDDM group were 0. 045μg/L and 8. 061 μg/L, both higher than that of healthy controls （0. 024μg/ L and 0. 832μg/L, P〈0.05）. The concentrations of HBD-3 were correlated positively with oral candida carriage （ rs =0. 245, P〈0.05）. The concentrations of HBD-2 and HBD-3 were also correlated positively （ rs = 0. 370, P〈0.01）. Conclusion： A higher prevalence of oral candidiasis and highercandida carriage were found in NIDDM patients compared with healthy controls. C. albicans was the most common isolated species. The levels of HBD-2, -3 in saliva were both up-regulated in NIDDM patients and were correlated positively with oral candida carriage.