中文摘要：

利用树枝状PAMAM大分子对电极表面进行修饰，戊二醛偶联固定抗原，并将羧基化的多壁碳纳米管偶联上二抗和辣根过氧化物酶制备多酶标记二抗复合物做检测信号，构建了具有双重放大效应的苯并[a]芘免疫传感器。在最优实验条件下，基于竞争法对苯并[a]芘实现了较好的检测性能，其线性范围为0．05—1．2μmol／L，检出限为0．02μmol／L。方法可应用于水体中苯并[a]芘的检测。

英文摘要：

In this work, a novel electrochemical was developed for the detection of benzo[a ] pyrene based on the dual signal amplification by using dendrimer-PAMAM to modify the electrode surface glutaraldehyde to couple the antigens, and multi-walled carbon nanotubes to associate secondary antibody and horseradish peroxidase as the detection signal. The experimental conditions were carefully optimized. Basing on a competitive immunoassay, benzo[ a ] pyrene competed with the immobilized antigen in the association ability of antibody, followed by the reaction with multi-enzyme-labeled second antibody. The results showed that the cathodic current change was linearly dependent on the logarithm of the benzo[ a ] pyrene concentration in the range of 0. 05 - 1.2 μmol/L with the detection limit of 0. 02 μmol/L. The proposed electrochemical immunosensor would be expected to gain widespread applications for the detection benzo[ a] pyrene in water.