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中文摘要：
用水热法合成表面修饰聚乙烯吡咯烷酮的硫化铋纳米粒子,借助氢键将其标记于5′端氨基修饰的寡聚核苷酸片段上,进而将其与固定于纳米金-碳糊电极上的目标DNA（来自于花椰菜花叶病毒的35 S启动子外源基因特定序列）进行杂交。用硝酸氧化溶解DNA杂交产物,以极谱络合吸附波测定溶解得到的Bi^3＋,成功实现了对目标DNA特定序列的检测,线性范围为1.0×10^-13～1.0×10^-8mol·L^-1,检测限达到3.8×10^-14mol·L^-1。此方法对1个碱基错配、互补和非互补序列具有很好的识别能力。
英文摘要：
Bismuth sulfide nanoparticle surface-modified with potyvinylpyrrolidone was synthesized under hydrothermal conditions, and used as a marker to label the DNA probe. Sequence-specific DNA related to the 35 S promoter was used as the target DNA sequence, which was immobilized covalently on carbon paste electrode modified with gold nanoparicles, and then hybridized with the probe DNA labeled with bismuth sulfide nanoparticle. The hybridizationevents were monitored by detection of Bi^3-via polarographic complex adsorptive wave. DNA specific-sequences were determined from 1.0×10^-13mol· L^-1 to 10^-8mol·L^-1 and a detection limit was 3.8×10^-14mol·L^-1 （S/N = 3）. The proposed method showed a distinguishable ability, reaching one base mismatched or the non-complementary DNA sequences with the complementary DNA sequences.
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