中文摘要：

电磁场对健康影响的研究包括流行病调查、人体与动物、细胞、生化与分子生物、生物物理等5个层次,电磁生物效应最初是通过物理作用产生化学反应,继而产生后续生物反应.自由基、电磁能量和生物钙是分属于化学、物理学和生物学的3个概念,研究它们之间的关系对于认识电磁生物效应的原初作用具有意义.选择海马神经元,观察在0.1mT、0.5mT和1.0mT电磁场暴露48h,海马神经元ROS水平和胞内Ca^2＋浓度的变化.实验结果表明：暴露于0.1mT,0.5mT和1.0mT电磁场海马神经元的ROS水平和Ca^2＋浓度都比对照组有显著性提高（P〈0.01）.暴露于0.1mT和0.5mT电磁场的ROS水平和暴露于0.1mT电磁场的Ca^2＋浓度与自由基清除剂＋电磁场（Trolox＋EMF）组比较没有差异（P〉0.05）,暴露于1.0mT电磁场的ROS水平和暴露于0.5mT和1.0mT电磁场的Ca^2＋浓度比Trolox＋EMF组有显著性提高（P〈0.01）.表明电磁场可以促进细胞自由基的产生,并且ROS水平与胞内Ca^2＋浓度有正相关性.

英文摘要：

The investigation of electromagnetic fields （EMF） in human health risk assessment include epidemiology, human and animals, cellular system, biochemistry and molecular biology, and biophysical mechanisms. The causal chain describes a series of sequential steps to lead to disease outcome for EMF to cause biological effect. To complete the first step, or say primary effect, the physical reaction between EMF with bio-molecules must come into being chemical reaction in organism, and then bring biological reaction. To research relationship of radical, energy and cytosolic calcium has significance to know primary effect of EMF. The effect of exposure to 48 h at 0.1mT, 0.5mT and 1.0mT power frequency EMF on rat-hippocampal neurons was investigated by evaluating the reactive oxygen species （ROS） and cytosolic Ca^2＋ concentration. The results show that the reactive oxygen species （ROS） and cytosolic Ca^2＋ concentration at 0.1mT, 0.5mT and 1.0 mT EMF have be all signification increaser （P〈0.01） than control （sham exposure）, ROS at 0.1mT and 0.5mT and Ca^2＋ at 0.1mT have be no difference （P〉0.05） than Trolox ＋ EMF, ROS at 1.0 mT and Ca^2＋ at 0.5mT and 1.0 mT have be signification increaser （P〈0.01） than Tolox ＋ EMF. It is known that EMF may enhance ROS formation in neurons, and there is positive correlation between ROS level and cytosolic Ca^2＋ concentration.