中文摘要：

(Rce1 ) 地岬和 a-factor-converting 酶 1 位于 endoplasmic 蜂窝胃(嗯) 并且被认为为绝大多数 CAAX 蛋白质处理的 endoproteolytic 负责。Endoplasmic 蜂窝胃应力(ERS ) 在肾的房间癌(RCC ) 起一个重要作用；然而，在 RCC 的 Rce1 的表示和角色广泛地没被学习。我们试图在 RCC 786-O 房间在嗯导致 apoptosis 在 RCC 纸巾和它的分子的机制调查 Rce1 的表示。我们首先使用了检测在肾的癌纸巾和 paracancerous 纸巾的 Rce1 表示的西方的弄污，量的反向的 transcriptase 聚合酶链反应，和 immunohistochemistry。Rce1 表示是在 RCC 的 upregulated，这被发现纸巾，和它的积极表示水平强烈与 clinicopathologic 特征被联系。下次，我们在人的胚胎的肾房间线 HEK293 和人的肾的癌房间线 786-O 检测了 Rce1 的表示， ACHN，和 A498。Rce1 的更高的表示在人的肾的癌房间线被发现，特别在 786-O 房间。在 786-O 房间的 Rce1 击倒增加的 apoptosis 和禁止的增长(P

英文摘要：

Ras and a-factor-converting enzyme 1 （Rcel） is located in the endoplasmic reticulum （ER） and is thought to be responsible for endoproteolytic processing of the vast majority of CAAX proteins. Endoplasmic reticulum stress （ERS） plays an important role in renal cell carcinoma （RCC）; however, the expression and role of Rcel in RCC have not been extensively studied. We aimed to investigate the expression of Rcel in RCC tissues and its molecular mechanism in ERS-induced apoptosis in RCC 786-0 cells. We first used western blotting, quantitative reverse transcriptase- polymerase chain reaction, and immunohistochemistry to detect the Rcel expression in renal carcinoma tissues and paracancerous tissues. It was found that Rcel expression was upregulated in RCC tissues, and its positive expression level was strongly associated with clinicopathologic features. Next, we detected the expression of Rcel in human embryonic kidney cell line HEK293 and human renal carcinoma cell lines 786-0, ACHN, and A498. Higher expression of Rcel was found in human renal carcinoma cell lines, especially in 786-0 cells. Knockdown of Rcel in 786-0 cells increased apoptosis and inhibited proliferation （P 〈 0.05）. Moreover, downregulation of Rcel upregulated the expression of the pro-apoptotic protein Bax, but downregulated the expression of the anti-apoptotic protein Bcl-2. Further studies showed that downregulation of Rcel also affected the expression of ERS factors. In conclusion, our results indicated that Rcel plays a key role in RCC. Low expression of Rcel might indirectly increase apoptosis and inhibit proliferation of renal carcinoma cells through ERS.