中文摘要：

目的研究外源性金属反应转录因子-1（metal—responsivetranscriptionfactor-1，MTF-1）对食管鳞癌ECl09细胞的增殖、凋亡、迁移及细胞周期的影响，并探讨其作用机制。方法构建MTF-1真核表达载体pcDNA3．1-MTF-1，转染ECl09细胞。采用RT-PCR和Westernblot分别检测MTF-1mRNA和蛋白水平的表达。采用CCK-8法检测细胞生长，流式细胞仪检测细胞凋亡和周期，Transwell实验检测细胞迁移能力。结果与转染pcDNA3．1的对照组相比，转染pcDNA3．1-MTF-1显著上调了ECl09细胞中MTF-1表达量。过表达MTF-148h后，ECl09细胞生长显著加快（P〈0．01）；细胞凋亡率降低（P〈0．05）；细胞周期比例发生显著改变，G1期细胞比例降低（P〈0．01），S期细胞比例升高（P〈0．01）；细胞迁移能力无显著改变。结论MTF-1通过抑制细胞凋亡、促进细胞周期G1到S期的转换进而促进食管鳞癌ECl09细胞的生长，但对ECl09的迁移能力没有显著影响。

英文摘要：

Objective To determine the effects of exogenous metal-responsive transcription factor-1 （MTF-1） on the cell proliferation, apoptosis, cell cycle and migration in human esophageal carcinoma ECI09 ceils. Methods The CDS fragment of MTF-1 was cloned into the pCDNA3.1 vector to construct recombinant vector pcDNA3.1-MTF-1. And then pcDNA3.1-MTF-1 and pcDNA3.1 （control） were transferred into EC109 cells separately. The mRNA and protein expression levels of MTF-1 were detected by RT-PCR and Western blotting. CCK8 assay was used to detect the effect of MTF-1 overexpression on EC109 cell proliferation and the effects on apoptosis and cell cycle were analyzed by flow cytometry. Transwell chamber assay was used to exam- ine the migration potential of the EC109 cells. Results Compared with pcDNA3.1 group, pcDNA3.1-MTF-1 group expressed high-level exogenous MTF-1 and the proliferation of EC109 cells were significantly increased in 48 to 96 h （P 〈0. 01 ）. After pcDNA3.1-MTF-1 transfection for 48 h, apoptosis of ECI09 cells was significantly inhibited （P 〈 0. 05 ）. Meanwhile, the percentage of EC109 cells in G1 phase was decreased （ P 〈 0. 01 ） and that in S phase was increased significantly （ P 〈 0. O1 ） as compared to the control group. The cell migration abilities didn＇t show significant difference between the 2 groups. Conclusion Exogenous MTF-1 stimulates the proliferation of EC109 cells by inhibiting apoptosis and promoting G1/S transition, while does not affect migration of ECI09 cells obviously.