从广东四会原始森林土壤中分离到一株能高效还原Fe(III)和腐殖质(HS)的兼性厌氧菌,编号为CY01,该菌株被鉴定为Comamonas koreensis(韩国丛毛单胞菌)。以Comamonas koreensis CY01为研究对象,研究了厌氧条件下HS(腐殖质)的模式物AQDS对微生物介导的2,4-D(2,4-二氯苯氧乙酸)还原脱氯过程的影响。研究结果显示,1)CY01具有使2,4-D进行脱氯降解的能力,CY01引起的2,4-D的脱氯降解过程是伴随着电子供体葡萄糖的氧化同时进行的;2)反应体系中加入AQDS,会促进2,4-D的降解过程,AQDS在Fe(III)/HS还原菌和2,4-D之间充当电子穿梭体。综合以上实验结果表明,CY01菌株引起的2,4-D的还原脱氯过程的完成是随着电子供体的氧化产生电子,然后AQDS将产生的电子加速传递至2,4-D,使2,4-D发生还原脱氯降解。
A facultative anaerobic bacterium which was a highly efficient humic substances and Fe(III)-reducing bacterium(designated CY01),was isolated from an ancient forest soil from Guangzhou,China and was identified as a strain of Comamonas koreensis.The role of the humic model compound,anthraquinone-2,6-disulfonate(AQDS),in the anaerobic reductive dechlorination of 2,4-dichlorophenoxyacetic acid(2,4-D) by C.koreensis CY01 was investigated.1) Strain CY01 could couple glucose oxidation to 2,4-D reductive dechlorination;2) Reductive dechlorination of 2,4-D by strain CY01 was greatly stimulated by the addition of AQDS which acts as an electron shuttle.2,4-D reduction proceeded by oxidation of electron donors and transference of electrons to the acceptors through the redox mediator,AQDS.