中文摘要：

目的血流动力学异常和心肌重塑是心肌梗死（MI）后室性心律失常形成的基础,本研究拟调查猪骨髓间充质干细胞（MSCs）移植对梗死心脏血流动力学和心肌重塑的影响。方法除正常对照组（Control组）外,MSCs移植组（MSCs组）和MI模型组（MI组）在完成MI动物模型制作时分别经左冠状动脉前降支移植MSCs悬液或等量生理盐水对照,6周后记录左室血流动力学参数值,检测心肌组织血管紧张素Ⅱ（AngⅡ）和胶原含量,并行心肌组织染色分析毛细血管密度和Ⅰ/Ⅲ型胶原比例。结果（1）MSCs组梗死区毛细血管密度显著高于MI组和Control组（P分别〈0.01）;（2）MSCs组血流动力学各参数值虽未恢复正常（同Control组相比,P分别〈0.01）,但较MI组均有显著改善（P分别〈0.05）;（3）MI组和MSCs组非梗死区心肌组织AngⅡ、胶原含量和Ⅰ/Ⅲ型胶原比例同Control组相比均有显著升高（P分别〈0.01）,但MSCs组心肌组织AngⅡ、胶原含量和Ⅰ/Ⅲ型胶原比例同MI组相比均有不同程度下降（P分别〈0.05、0.01和0.01）。结论MSCs移植能显著增加梗死区毛细血管网重建,改善血流动力学状况,减轻心肌重塑程度,有助于减少室性心律失常的基质形成。

英文摘要：

Objective To investigate the effects of mesenchymal stem cell（MSC） transplantation in post-infarcted swine myocardium on hemodynamics and myocardial remodeling. Methods Ten swine were used as normal controls（Control group）, 23 swine with reperfused acute myocardial infarction（AMI） received intracoronary infusion of either MSC solution（ MSC group, n = 13） or 0.9% sodium chloride solution（ MI group, n = 10）. Six weeks later, the levels of hemodynamic parameters, density of capillaries, concentrations of angiotensin II and collagen in non-infarcted myocardium, and ratio of collagen type I and m were detected. Results （1） The capillary density of peri-infarct area in the MSC group was markedly higher than that in the Control and MI group（ all P 〈 0.01） ; （2） The levels of hemedynamic parameters in the MSC group, although didn＇t reach the normal levels showed in the Control group, improved significantly compared with those in the MI group（all P〈0.05）. （3） The concentrations of angiotensin II and collagen, and ratio of collagen typeIand Ⅲ in the non-infarct area in MSC group were markedly higher than those in the Control group（all P〈0.01） and, however, markedly lower than those in the MI group（P 〈 0.05, 0.01 and 0.01, respectively）. Conclusion MSC-based therapy can improve impaired cardiac performances, lessen the degree of myocardial remodeling and, thus, reduce the risk of ventricular arrhythmias after MI.