中文摘要：

目的通过小干扰RNA沉默检测DDX39基因对肾透明细胞癌增殖的影响及其相关机制。方法应用脂质体转染法将DDX39-siRNA转染人肾透明细胞癌786-O细胞,试验设空白对照组、阴性对照组、小干扰RNA转染组（DDX39-siRNA）。应用实时荧光定量PCR和Western印迹法检测DDX39-siRNA的干扰效率;MTT法和平板克隆形成实验检测DDX39对786-O细胞增殖的影响;Western印迹法检测细胞增殖指标PCNA;流式细胞术检测DDX39-siRNA干扰后786-O细胞周期的变化。结果与阴性对照组相比,转染DDX39-siRNA后,786-O细胞中DDX39 mRNA及其蛋白的表达水平明显下降,差异有统计学意义（P〈0.05）;DDX39-siRNA干扰后786-O细胞增殖能力受到抑制,克隆形成能力明显下降;S期细胞减少,G2/M期细胞百分比明显增加（P〈0.05）。结论 DDX39敲减后可显著抑制肾癌细胞786-O细胞增殖,并阻滞细胞于G2/M期。

英文摘要：

Objective To investigate the effect of DDX39 on human renal carcinoma 786-O cells by siRNA silence. Methods The DDX39-siRNA targetinghuman DDX39 gene was transfected into786-O cells by Super FectinTMⅡ in vitrosiRNA transfection reagent. The cells were divided intoblank control group,negative control group and siRNA interference group（ DDX39-siRNA）. The expression of DDX39 mRNA and protein was detected by Real-Time PCR and Western blotting,respectively. The cell proliferation was measured by M TTmethod and colony formation. The proliferation protein marker was detected by Western blottingand the cell cycle was detected by flowcytometry. Results As compared with the negative control,the expression level of DDX39 mRNA and protein level was decreased after transfection of DDX39-siRNA（ P〈0. 05）. The proliferation of 786-O cells was declined and the ability of colony-formation was decreased. The cell cycle of 786-O cells was arrested at G2/ M phase and S phase was decreased（ P〈0. 05）. Conclusion DDX39-siRNA can down regulate expression levels of DDX39 mRNA and protein in human renal cancer 786-O cells,resultingin the inhibition of cell proliferation and cell cycle.