中文摘要：

为了深入研究芥菜开花整合子SOC1基因的表达调控机制,利用染色体步移法从芥菜‘QJ’中克隆了SOC1编码区上游782 bp的启动子,并构建SOC1基因启动子的酵母表达载体p Ab Ai-SOC1,与蛋白表达载体p GADT7-FLC、p GADT7-SVP共转化酵母Y1HGold菌株。酵母单杂交表明：芥菜FLC和SVP蛋白均能与SOC1的启动子相互作用。进一步分析发现：SOC1启动子含3个CAr G-box表达调控基序。分别亚克隆这3个基因片段（SOC1-1、SOC1-2和SOC1-3）,并再次构建酵母重组质粒p Ab Ai-SOC1-1、pA b Ai-SOC1-2和p Ab Ai-SOC1-3,与p GADT7-FLC、p GADT7-SVP分别融合到Y1HGold菌株。融合菌株均能在相应SD/-Leu/Ab A培养基上生长,说明SOC1-1、SOC1-2和SOC1-3都能被芥菜FLC、SVP蛋白识别并结合。再次构建SOC1-1、SOC1-2、SOC1-3的CAr G-box删除突变体及A-T互换突变体,则均不能与FLC、SVP蛋白互作。由此说明：SOC1-1、SOC1-2和SOC1-3的3个CAr G-box基序确实能特异性识别FLC、SVP,发生DNA—蛋白相互作用。这为利用启动子调控SOC1基因的转录表达等深入研究奠定了理论基础。

英文摘要：

In order to clarify the expression regulation mechanism of the flowering signal integrator SOC1 gene,a 782 bppromoter of SOC1 gene was cloned from Brassica juncea‘QJ＇via genome walking kit. Yeast promoter-reporter vector pAb Ai was constructed with SOC1 promoter and transformed into Y1 HGold strain. Then protein vectors,pGADT7-FLC and pGADT7-SVP,were transformed into Y1HGold（pAb Ai-SOC1）strain,respectively,to test the interactions of SOC1 promoter with FLC and SVPvia yeast one hybrid system. The results showed that Brassica juncea FLC and SVP proteins could interact with SOC1 promoter,respectively. Further analysis indicated that SOC1 promoter had three CAr G-box moitfs,which probably regulated the DNA-protein interactions. Thus,three fragments of SOC1 promoter were subcloned respectively and named SOC1-1,SOC1-2 and SOC1-3,each of which had a single CAr G-box. The plasmids,pAb Ai-SOC1-1,pAb Ai-SOC1-2 and pAb Ai-SOC1-3,were also constructed,and then transformed into Y1 HGold strain and fused with pGADT7-FLC and pGADT7-SVP plasmids,respectively. All the zygote strains could grow on the SD/-Leu/Ab A plates,suggesting that SOC1-1,SOC1-2 and SOC1-3 could be targeted by Brassica juncea FLC and SVP proteins,respectively. To verify the specificity of the interactions,we additionally constructed three mutants which deleted their whole CAr G-box,as well as another three mutants that only exchanged A and T in CAr G-box. However,the DNA–protein interactions vanished after CAr G-box was deleted or mutated,which suggests that the three CAr G-box motifs in SOC1-1,SOC1-2 and SOC1-3 could specifically interact with FLC and SVP proteins. The study provided valuable information for further studies on the transcriptional expression regulation of SOC1 gene in flowering-time control.