中文摘要：

目的：探讨全反式维甲酸（ATRA）对chemerin基因表达的调控并初探其调控机制。方法：培养HepG2细胞,加入全反式维甲酸,观察其对chemerin表达的影响。采用实时PCR检测chemerin在mRNA水平的表达变化;蛋白质印迹法检测chemerin在蛋白水平的表达变化。通过构建含不同长度chemerin启动子区域的报告质粒,用检测荧光素酶活性的方法,研究ATRA与维甲酸受体（RAR）β结合对chemerin启动子区域的影响。结果：ATRA可呈量效关系诱导HepG2细胞chemerin的mRNA及蛋白水平的表达升高,在10μmol/L时作用最强（P〈0.05）;呈时效关系诱导HepG2细胞chemerin的蛋白水平表达升高,在作用36 h时诱导作用最强（P〈0.05）;去除chemerin启动子-258bp/＋121bp区后,ATRA对chemerin启动子的转录激活作用骤然下降,提示ATRA-RARβ与chemerin启动子的-258bp/-90bp区域结合。结论：ATRA可上调HepG2细胞chemerin的表达,RARβ介导ATRA对chemerin启动子区的转录激活作用。

英文摘要：

Objective： To investigate the effect and mechanism of all-trans retinoic acid（ATRA） in regulating the expression of chemerin.Methods： HepG2 cells were treated with ATRA in different doses and time.Chemerin mRNA expression level was examined by real-time PCR and Western blotting was used to analyze the expression of chemerin protein.Four consecutive fragments of the chemerin promoter region were individually inserted into reporter plasmid.A luciferase assay was used to study the effect of ATRA on chemerin promoter region.Results：Treating HepG2 cells with ATRA caused dose-dependent increase in the expression of chemerin mRNA and protein.Levels of chemerin reached a maximum level of about two-fold of control at a concentration of 10 μmol/L.Treating HepG2 cells with ATRA also caused time-dependent increase in the expression of chemerin protein,and caused increase in the expression of chemerin mRNA.The expression of chemerin protein increased markedly 36 h after the addition to the cells of ATRA at a concentration of 10 μmol/L.Deletion of the-258bp to ＋121bp region of the chemerin promoter sharply reduced the transactivation efficiency by ATRA-RARβ,suggesting a high possibility that ATRA-RARβ regulation site was within-258 bp/-90 bp region.Conclusion： ATRA increased the expression of chemerin,and RARβ mediated ATRA-induced chemerin expression.