本文从2009到2011年连续3年用马拉硫磷、毒死蜱、灭多威、三氟氯氰菊酯、吡虫啉、硫丹6种杀虫药剂诊断剂量监测了河北、河南、山东、安徽等重要棉产区绿盲蝽Apolygus lucorum(Meyer-Dür)田间种群的抗药性。2010年马拉硫磷诊断剂量处理河北邱县、安徽望江田间种群死亡率95%以上;毒死蜱诊断剂量河南郑州、山东滨州,安徽地区的田间绿盲蝽死亡率大于80%;三氟氯氰菊酯诊断剂量死亡率接近95%以上;2010年安徽望江、2011年河北邱县、山东滨州、安徽无为田间种群在吡虫啉诊断剂量下的死亡率在78%~90%。点滴法对2011年各地绿盲蝽种群抗药性监测表明,各地绿盲蝽对毒死蜱、灭多威、吡虫啉均处于敏感阶段,对于马拉硫磷、三氟氯氰菊酯、硫丹大多处于低抗水平阶段。在对中黑盲蝽Adelphocoris suturalis Jakovlev3年的抗性监测中,作者发现种群抗性个体频率的出现更为普遍。
Effective doses of the insecticides,malathion,chlorpyrifos,methomyl,lambda-cyhalothrin,imidacloprid,endosulfan,to control Apolygus lucorum (Meyer-Dür) and Adelphocoris suturalis Jakovlev were determined based on the susceptible base-line of both species.Resistance of both A.lucorum and A.suturalis to the above insecticides was monitored with the diagnostic dose method in cotton fields in Hebei,Henan,Shangdong and Anhui provinces.The diagnostic dose of malathion resulted in more than 95% motality in field populations of Hebei's Qiu County and Anhui's Wangjiang County in 2010.Diagnostic doses of chlorpyrifos resulted in more than 80% mortality in field populations in Henan's Zhengzhou and Shandong's Binzhou.All populations of the green plant bug,collected from Hebei,Henan,Shandong and Anhui provinces were susceptible to methomyl and lambda-cyhalothrin.The mortality of field populations after exposure to diagnose doses of imidacloprid ranged from 78% to 90% in Anhui's Wangjiang County in 2010,and in Hebei's Qiu County,Shandong's Binzhou County and Anhui's Wuwei County in 2011.Bioassay of the effects of topical application of these pesticides revealed that populations of the green plant bug were susceptible to chlorpyrifos,methomyl and imidacloprid,and possessed lower resistance to malathion,lambda-cyhalothrin and endosulfan in the above provinces.Our results demonstrate that the diagnostic dose method of monitoring insecticide resistance is both reliable and convenient.