以3种常见冷季型草坪草——白三叶(Trifolium repens)、草地早熟禾(Poa pratensis)和苇状羊茅(Festuca arundinacea)为实验材料,利用开顶箱(OTC)模拟法,研究了这些草坪草在高浓度O3(80和120 nmol·mol-1)处理下生理生态指标的变化。结果表明:3种草坪草随O3胁迫加剧,呈现不同的伤害症状,其中,白三叶在熏蒸前期最先出现黄褐色点状伤斑。高浓度O3显著抑制了3种草坪草植株的生长。与对照相比,高浓度O3处理下显著提高了3种草坪草叶片中丙二醛(MDA)含量及SOD活性(88%、74%、47%)与POD活性(136%、5%、9%)(P〈0.05)。草地早熟禾与苇状羊茅的脱落酸(ABA)含量随O3浓度升高而升高,而白三叶的ABA含量则呈现出先升高后降低的趋势。研究表明:白三叶对高浓度O3最为敏感,可用于做臭氧的指示植物;苇状羊茅对O3的敏感性最低,抗性最强。
An experiment was conducted to study the variations of the physiological indexes in leaves of Trifolium repens,Poa pratensis and Festuca arundinacea exposed to elevated O3 concentrations( 80 and 120 nmol · mol-1) in open top chambers( OTCS). The results showed that three turfgrass species showed different injury symptoms induced by O3 stress,while brown injury spots appeared on T. repens first. Elevated O3 significantly inhibited the growth of the three grass species and significantly increased the content of malondialdehyde( MDA) and the activities of superoxide dismutase( SOD) and peroxidase( POD)( P 〈 0. 05). Elevated O3 significantly increased the content of abscisic acid( ABA) in leaves of P. pratensis and F. arundinacea,while T. repens showed a decreasing trend after an initial increase. The results showed that T. repens is most sensitive to high concentrations of O3 while F. arundinacea least.